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Authordc.contributor.authorReyes S., Marcelo 
Authordc.contributor.authorTorres Torretti, Juan Pablo 
Authordc.contributor.authorPrado Jiménez, Valeria 
Authordc.contributor.authorVidal A., Roberto 
Cita de ítemdc.identifier.citationRevista Medica de Chile, Volumen 136, Issue 3, 2018, Pages 338-346
Abstractdc.description.abstractBackground: Acute bacterial meningitis (ABM) is a serious disease that needs rapid diagnosis for an accurate treatment. The most important etiological agents are: Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae type b. Overall pathogen detection rate in patients with ABM in Chile is 83%. Aim: To evaluate a Polymerase Chain Reaction (PCR) protocol for simultaneous detection of several pathogens in patients with ABM. Material and methods: We designed and evaluated a multiplex PCR protocol for simultaneous specific genes identifications of S pneumoniae (lytA and ply genes), N meningitidis (ctrA, crgA) and H influenzae (bexA) in cerebrospinal fluid (CSF) samples from pediatric patients with suspected diagnosis of ABM. Sensitivity, specificity and minimum detection levels of DNA were determined. Amplifications of rDNA 16S gene was done to confirm extraction of bacterial DNA. Results: Ninety nine CSF samples were studied, 90 from children with fever and negative C
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.uri
Sourcedc.sourceRevista Medica de Chile
Keywordsdc.subjectHemophilus influenzae
Keywordsdc.subjectMeningitis, bacterial
Keywordsdc.subjectMeningitis, Streptococcus pneumoniae
Keywordsdc.subjectNeisseria meningitidis
Keywordsdc.subjectPolymerase chain reaction
Títulodc.titleMultiplex PCR assay in spinal fluid to identify simultaneously bacterial pathogens associated to acute bacterial meningitis in Chilean children Diseño y evaluación de una reacción de polimerasa en cadena (RPC) múltiple, para la identificación de bacterias
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación SCOPUS

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile