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Nuclear factor κB pathway and interleukin-6 are affected in eutopic endometrium of women with endometriosis
Autor | dc.contributor.author | Ponce, Carlos | |
Autor | dc.contributor.author | Torres, Marisa | |
Autor | dc.contributor.author | Galleguillos, Carolina | |
Autor | dc.contributor.author | Sovino, Hugo | |
Autor | dc.contributor.author | Boric Scarpa, María Angélica | |
Autor | dc.contributor.author | Fuentes, Ariel | |
Autor | dc.contributor.author | Johnson, M. Cecilia | |
Fecha ingreso | dc.date.accessioned | 2019-03-11T12:58:06Z | |
Fecha disponible | dc.date.available | 2019-03-11T12:58:06Z | |
Fecha de publicación | dc.date.issued | 2009 | |
Cita de ítem | dc.identifier.citation | Reproduction, Volumen 137, Issue 4, 2018, Pages 727-737 | |
Identificador | dc.identifier.issn | 14701626 | |
Identificador | dc.identifier.issn | 17417899 | |
Identificador | dc.identifier.other | 10.1530/REP-08-0407 | |
Identificador | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/164811 | |
Resumen | dc.description.abstract | In order to investigate the role of the nuclear factor κB (NFKB) pathway on gene expression in the eutopic endometrium in endometriosis, and in particular of interleukin-6 (IL6), we evaluated RELA, IκB kinase (CHUK), NFKBIA and IL6 expressions and NFKB DNA binding in eutopic endometrium from women with endometriosis. Eutopic endometrium was obtained from 37 women with endometriosis and 42 fertile women during laparoscopy. We analysed RELA, CHUK, NFKBIA and IL6 mRNA levels (RT-PCR); RELA, CHUK and NFKBIA proteins and p-NFKBIA/NFKBIA ratio (western blot); and NFKB binding (DNA shift assay) and IL6 concentration (ELISA) in endometrial explants. Our results indicate that mRNA and cytoplasmic proteins of RELA and CHUK exhibit constant levels in normal endometrium during the menstrual cycle. A dramatic increase (P<0.05) in NFKBIA mRNA expression, RELA nuclear presence and the mRNA and the protein of IL6 during late secretory phase was also observed in this tissue. By contrast, in eutopic end | |
Idioma | dc.language.iso | en | |
Tipo de licencia | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
Link a Licencia | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
Fuente | dc.source | Reproduction | |
Palabras claves | dc.subject | Reproductive Medicine | |
Palabras claves | dc.subject | Embryology | |
Palabras claves | dc.subject | Endocrinology | |
Palabras claves | dc.subject | Obstetrics and Gynecology | |
Palabras claves | dc.subject | Cell Biology | |
Título | dc.title | Nuclear factor κB pathway and interleukin-6 are affected in eutopic endometrium of women with endometriosis | |
Tipo de documento | dc.type | Artículo de revista | |
dcterms.accessRights | dcterms.accessRights | Acceso Abierto | |
Catalogador | uchile.catalogador | SCOPUS | |
Indización | uchile.index | Artículo de publicación SCOPUS | |
uchile.cosecha | uchile.cosecha | SI |
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