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Molecular characterization of IPNV RNA replication intermediates during the viral infective cycle
Autor | dc.contributor.author | Cortez-San Martín, Marcelo | |
Autor | dc.contributor.author | Villanueva, Rodrigo A. | |
Autor | dc.contributor.author | Jashés, Matilde | |
Autor | dc.contributor.author | Sandino, | |
Fecha ingreso | dc.date.accessioned | 2019-03-11T12:58:09Z | |
Fecha disponible | dc.date.available | 2019-03-11T12:58:09Z | |
Fecha de publicación | dc.date.issued | 2009 | |
Cita de ítem | dc.identifier.citation | Virus Research, Volumen 144, Issue 1-2, 2018, Pages 344-349 | |
Identificador | dc.identifier.issn | 01681702 | |
Identificador | dc.identifier.other | 10.1016/j.virusres.2009.05.007 | |
Identificador | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/164822 | |
Resumen | dc.description.abstract | Infectious Pancreatic Necrosis Virus (IPNV) is a bisegmented, double-stranded RNA virus, which belongs to the Birnaviridae family. In the current study, we have analyzed the RNA replication intermediates (RI) purified throughout the viral replication cycle in cultured cells. Equilibrium ultracentrifugation of infected cellular lysates resulted in two major peaks of viral components. The first peak, at a buoyant density of 1.33 g/cm3, contained assembled IPNV viral particles A and B, whereas the second peak, located at buoyant densities >1.4 g/cm3, contained a higher molecular weight viral ribonucleoprotein complex composed of, at least, VPg/VP1 and a heterogeneous population of single- and double-stranded viral RNA species. Interestingly, analyses of these dsRNA RI indicated that they contain single-stranded segments of incompletely synthesized positive-strands of RNA. Northern blot experiments of total RNA isolated from infected cells confirmed our proposed configuration of the RNA RI | |
Idioma | dc.language.iso | en | |
Tipo de licencia | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
Link a Licencia | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
Fuente | dc.source | Virus Research | |
Palabras claves | dc.subject | dsRNA synthesis | |
Palabras claves | dc.subject | Genome replication intermediates | |
Palabras claves | dc.subject | Infectious Pancreatic Necrosis Virus | |
Palabras claves | dc.subject | IPNV | |
Palabras claves | dc.subject | Replication mechanism | |
Título | dc.title | Molecular characterization of IPNV RNA replication intermediates during the viral infective cycle | |
Tipo de documento | dc.type | Artículo de revista | |
Catalogador | uchile.catalogador | SCOPUS | |
Indización | uchile.index | Artículo de publicación SCOPUS | |
uchile.cosecha | uchile.cosecha | SI |
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