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Autordc.contributor.authorCortez-San Martín, Marcelo 
Autordc.contributor.authorVillanueva, Rodrigo A. 
Autordc.contributor.authorJashés, Matilde 
Autordc.contributor.authorSandino, 
Fecha ingresodc.date.accessioned2019-03-11T12:58:09Z
Fecha disponibledc.date.available2019-03-11T12:58:09Z
Fecha de publicacióndc.date.issued2009
Cita de ítemdc.identifier.citationVirus Research, Volumen 144, Issue 1-2, 2018, Pages 344-349
Identificadordc.identifier.issn01681702
Identificadordc.identifier.other10.1016/j.virusres.2009.05.007
Identificadordc.identifier.urihttps://repositorio.uchile.cl/handle/2250/164822
Resumendc.description.abstractInfectious Pancreatic Necrosis Virus (IPNV) is a bisegmented, double-stranded RNA virus, which belongs to the Birnaviridae family. In the current study, we have analyzed the RNA replication intermediates (RI) purified throughout the viral replication cycle in cultured cells. Equilibrium ultracentrifugation of infected cellular lysates resulted in two major peaks of viral components. The first peak, at a buoyant density of 1.33 g/cm3, contained assembled IPNV viral particles A and B, whereas the second peak, located at buoyant densities >1.4 g/cm3, contained a higher molecular weight viral ribonucleoprotein complex composed of, at least, VPg/VP1 and a heterogeneous population of single- and double-stranded viral RNA species. Interestingly, analyses of these dsRNA RI indicated that they contain single-stranded segments of incompletely synthesized positive-strands of RNA. Northern blot experiments of total RNA isolated from infected cells confirmed our proposed configuration of the RNA RI
Idiomadc.language.isoen
Tipo de licenciadc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link a Licenciadc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Fuentedc.sourceVirus Research
Palabras clavesdc.subjectdsRNA synthesis
Palabras clavesdc.subjectGenome replication intermediates
Palabras clavesdc.subjectInfectious Pancreatic Necrosis Virus
Palabras clavesdc.subjectIPNV
Palabras clavesdc.subjectReplication mechanism
Títulodc.titleMolecular characterization of IPNV RNA replication intermediates during the viral infective cycle
Tipo de documentodc.typeArtículo de revista
Catalogadoruchile.catalogadorSCOPUS
Indizaciónuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
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