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Authordc.contributor.authorAvalos, Ana María 
Authordc.contributor.authorValdivia, Alejandra D. 
Authordc.contributor.authorMuñoz, Nicolás 
Authordc.contributor.authorHerrera Molina, Rodrigo 
Authordc.contributor.authorTapia, Julio C. 
Authordc.contributor.authorLavandero González, Sergio 
Authordc.contributor.authorChiong Lay, Mario 
Authordc.contributor.authorBurridge, Keith 
Authordc.contributor.authorSchneider, Pascal 
Authordc.contributor.authorQuest, Andrew F. G. 
Authordc.contributor.authorLeyton Campos, Lisette 
Cita de ítemdc.identifier.citationJournal of Cell Science, Volumen 122, Issue 19, 2018, Pages 3462-3471
Abstractdc.description.abstractClustering of αvβ3 integrin after interaction with the RGD-like integrin-binding sequence present in neuronal Thy-1 triggers formation of focal adhesions and stress fibers in astrocytes via RhoA activation. A putative heparin-binding domain is present in Thy-1, raising the possibility that this membrane protein stimulates astrocyte adhesion via engagement of an integrin and the proteoglycan syndecan-4. Indeed, heparin, heparitinase treatment and mutation of the Thy-1 heparin-binding site each inhibited Thy-1-induced RhoA activation, as well as formation of focal adhesions and stress fibers in DI TNC1 astrocytes. These responses required both syndecan-4 binding and signaling, as evidenced by silencing syndecan-4 expression and by overexpressing a syndecan-4 mutant lacking the intracellular domain, respectively. Furthermore, lack of RhoA activation and astrocyte responses in the presence of a PKC inhibitor or a dominant-negative form of PKCα implicated PKCα and RhoA activation in these e
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
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Sourcedc.sourceJournal of Cell Science
Keywordsdc.subjectCell adhesion
Títulodc.titleNeuronal Thy-1 induces astrocyte adhesion by engaging syndecan-4 in a cooperative interaction with αvβ3 integrin that activates PKCα and RhoA
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación SCOPUS

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