Clues to understanding cold sensation: Thermodynamics and electrophysiological analysis of the cold receptor TRPM8
Author
dc.contributor.author
Brauchi, Sebastián
Author
dc.contributor.author
Orio, Patricio
Author
dc.contributor.author
Latorre, Ramón
Admission date
dc.date.accessioned
2019-03-11T12:59:06Z
Available date
dc.date.available
2019-03-11T12:59:06Z
Publication date
dc.date.issued
2004
Cita de ítem
dc.identifier.citation
Proceedings of the National Academy of Sciences of the United States of America, Volumen 101, Issue 43, 2018, Pages 15494-15499
Identifier
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00278424
Identifier
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10.1073/pnas.0406773101
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/164922
Abstract
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The cold and menthol receptor, TRPM8, also designated CMR1, is a member of the transient receptor potential (TRP) family of excitatory ion channels. TRPM8 is a channel activated by cold temperatures, voltage, and menthol. In this study, we characterize the cold-and voltage-induced activation of TRPM8 channel in an attempt to identify the temperature- and voltage-dependent components involved in channel activation. Under equilibrium conditions, decreasing temperature has two effects. (i) It shifts the normalized conductance vs. voltage curves toward the left, along the voltage axis. This effect indicates that the degree of order is higher when the channel is in the open configuration. (ii) It increases the maximum channel open probability, suggesting that temperature affects both voltage-dependent and -independent pathways. In the temperature range between 18°C and 25°C, large changes in enthalpy (ΔH = -112 kcal/mol) and entropy (ΔS = -384 cal/mol K) accompany the activation process. Th