Rat spermatogenesis damage in intermittent hypobaric hypoxia and the protective role of melatonin. II: Testicular parameters Daño de la espermatogénesis en rata en la hipoxia hipobárica intermitente y rol protector de la melatonina. II: Parámetros testicu
Author
dc.contributor.author
Bustos Obregón, Eduardo
Author
dc.contributor.author
Castro-Sánchez, Rodrigo
Author
dc.contributor.author
Ramos-González, Benito
Author
dc.contributor.author
Torres Díaz, Leandro
Admission date
dc.date.accessioned
2019-03-11T13:00:16Z
Available date
dc.date.available
2019-03-11T13:00:16Z
Publication date
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2010
Cita de ítem
dc.identifier.citation
International Journal of Morphology, Volumen 28, Issue 2, 2018, Pages 537-547
Identifier
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07179502
Identifier
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07179367
Identifier
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10.4067/S0717-95022010000200034
Identifier
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https://repositorio.uchile.cl/handle/2250/165078
Abstract
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At present it is not clear if male fertility is affected by intermittent hypobaric hypoxia (IHH). This is an important issue since a large human population works over 3000 masl. This study analyzes testicular changes in adult Sprague Dawley rats after five cycles of IHH (7 day exposure to 4200 masl in a hypobaric chamber / 7 day at 500 masl). The animals were separated into groups of 8, one group was exposed to hypoxia (7 days), and the others to IHH for one to five cycles. Controls (500 masl) were examined at the beginning and at the end of the 70 experimental days. A duplicate set of rats treated with melatonin (supposedly protecting from hypoxia) was also examined, as were their controls, injected with 0,03% ethanol (melatonin solvent).Immunohistochemical and histometric analysis of testicular tissue were performed. Damage caused by IHH increases with time. Morphometry reveals an increase in tubular and luminal diameters and a reduction in epithelial height. Inmunohistochemistry for
Rat spermatogenesis damage in intermittent hypobaric hypoxia and the protective role of melatonin. II: Testicular parameters Daño de la espermatogénesis en rata en la hipoxia hipobárica intermitente y rol protector de la melatonina. II: Parámetros testicu