P450Arom induction in isolated control endometrial cells by peritoneal fluid from women with endometriosis

Abstract

Objective: To study the effect of peritoneal fluid from women with (PF-E) and without (PF-C) endometriosis on P450Arom expression in endometrial cells. Design: Experimental study. Setting: University research unit. Patient(s): Forty women of reproductive age with (n ¼ 22) or without (control; n ¼ 18) endometriosis. Intervention(s): Peritoneal fluid and eutopic endometrial samples were obtained during surgery from women with (n ¼ 13 and 9, respectively) and without (n ¼ 4 and 14, respectively) endometriosis. Main Outcome Measure(s): Expression study for P450Arom, steroid factor 1 (SF-1), chicken ovalbumin upstream transcription factor I (COUP-TFI), and COUP-TFII messenger RNA (reverse transcriptase–polymerase chain reacion) and/or protein (immunoblot) in isolated endometrial epithelial cells transfected or not with expression vector containing SF-1, COUP-TFI, or COUP-TFII complementary DNAs. Result(s): Basal messenger RNA and/or protein expression of P450Arom and SF-1 were augmented in endometriosis, and that of COUP-TF was diminished. In control cells, (Bu)2cAMP and PF-E increased P450Arom and SF-1 expression (but not COUP-TF expression) in a dose-dependent way, an effect not observed with PF-C, adsorbed PF-E, or 10 5 M indomethacin. Transfected cells confirmed these results. Any treatments modified the studied molecules in endometriosis cells. Conclusion(s): These data indicate that molecules contained in PF-E favor an estrogenic microenvironment, suggesting a role in the etiopathogenesis of endometriosis enabling the survival, maintenance, and growth of endometrial implants in the ectopic locations.