Role of the α-adrenergic system in femoral vascular reactivity in neonatal llamas and sheep: A comparative study between highland and lowland species

Abstract

Using an integrative approach at the whole animal, isolated vessels, and molecular levels, we tested the hypothesis that the llama, a species that undergoes pregnancy under the influence of the chronic hypoxia of high altitude, delivers offspring with an increased α-adrenergic peripheral vascular reactivity compared with neonates from lowland species. We studied the femoral vascular response to acute hypoxia in vivo, the reactivity of femoral vessels ex vivo, and the expression of femoral α 1-adrenergic receptor subtypes using RT-PCR in vitro. The increase in femoral resistance during hypoxia was 3.6 times greater in newborn llamas than newborn sheep (P<0.05). The sensitivity of the contractile response to noradrenaline (pD2=5.18±0.06 vs. 4.84±0.05, P < 0.05) and the maximal response (R max=101.3±1.4 vs.52.4±1.4% K + max, P < 0.05) and sensitivity (pD 2 = 5.47 ± 0.03vs. 4.57 ± 0.05, P<0.05) to phenylephrine were higher in femoral vessels from newborn llamas than newborn sheep. Competit