DAX-1 and DAX-1A expression in human testicular tissues with primary spermatogenic failure
Author
dc.contributor.author
Lardone, M. C.
Author
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Parada-bustamante, A.
Author
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Ebensperger, M.
Author
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Valdevenito, R.
Author
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Kakarieka, E.
Author
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Martínez, D.
Author
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Pommer, R.
Author
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Piottante, A.
Author
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Castro, A.
Admission date
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2019-03-11T13:02:57Z
Available date
dc.date.available
2019-03-11T13:02:57Z
Publication date
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2011
Cita de ítem
dc.identifier.citation
Molecular Human Reproduction, Volumen 17, Issue 12, 2018, Pages 739-746
Identifier
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13609947
Identifier
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14602407
Identifier
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10.1093/molehr/gar051
Identifier
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https://repositorio.uchile.cl/handle/2250/165408
Abstract
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DAX-1 [dosage-sensitive sex reversal-adrenal hypoplasia congenital (AHC) critical region on the X chromosome gene 1; NR0B1] is an orphan nuclear receptor that acts as a transcriptional repressor in adrenal/gonadal development, steroidogenesis and probably spermatogenesis. An alternatively spliced form called DAX-1A (NR0B1A) has been described in several tissues including the testis, and in vitro studies have shown an inhibitory effect on DAX-1 transcriptional function. We aimed to study the mRNA and protein expression of DAX-1 in testicular tissues of 65 men with primary spermatogenic failure [complete Sertoli cell only syndrome (SCOS), focal SCOS, maturation arrest and mixed atrophy] compared with 33 controls with normal spermatogenesis. As a novel finding, we observed intense immunostaining, not only in the nucleus of Sertoli cells, but also in pachytene spermatocytes and round spermatids. The quantitative mRNA expression of DAX-1 and DAX-1A was similar between cases and controls and