Astrocytic α vβ 3 integrin inhibits neurite outgrowth and promotes retraction of neuronal processes by clustering thy-1
Author
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Herrera Molina, Rodrigo
Author
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Frischknecht, Renato
Author
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Maldonado, Horacio
Author
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Seidenbecher, Constanze I.
Author
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Gundelfinger, Eckart D.
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Hetz Flores, Claudio
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Aylwin Ostale, María de la Luz
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Schneider, Pascal
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Quest, Andrew F. G.
Author
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Leyton Campos, Lisette
Admission date
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2019-03-11T13:02:57Z
Available date
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2019-03-11T13:02:57Z
Publication date
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2012
Cita de ítem
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PLoS ONE, Volumen 7, Issue 3, 2018,
Identifier
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19326203
Identifier
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10.1371/journal.pone.0034295
Identifier
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https://repositorio.uchile.cl/handle/2250/165410
Abstract
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Thy-1 is a membrane glycoprotein suggested to stabilize or inhibit growth of neuronal processes. However, its precise function has remained obscure, because its endogenous ligand is unknown. We previously showed that Thy-1 binds directly to α Vβ 3 integrin in trans eliciting responses in astrocytes. Nonetheless, whether α Vβ 3 integrin might also serve as a Thy-1-ligand triggering a neuronal response has not been explored. Thus, utilizing primary neurons and a neuron-derived cell line CAD, Thy-1-mediated effects of α Vβ 3 integrin on growth and retraction of neuronal processes were tested. In astrocyte-neuron co-cultures, endogenous α Vβ 3 integrin restricted neurite outgrowth. Likewise, α Vβ 3-Fc was sufficient to suppress neurite extension in Thy-1(+), but not in Thy-1(-) CAD cells. In differentiating primary neurons exposed to α Vβ 3-Fc, fewer and shorter dendrites were detected. This effect was abolished by cleavage of Thy-1 from the neuronal surface using phosphoinositide-specific