Show simple item record

Authordc.contributor.authorCastillo, Juan P. 
Authordc.contributor.authorDe Giorgis, Daniela 
Authordc.contributor.authorBasilio, Daniel 
Authordc.contributor.authorGadsby, David C. 
Authordc.contributor.authorRosenthal, Joshua J.C. 
Authordc.contributor.authorLatorre, Ramón 
Authordc.contributor.authorHolmgren, Miguel 
Authordc.contributor.authorBezanill, Francisco 
Cita de ítemdc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, Volumen 108, Issue 51, 2018, Pages 20556-20561
Abstractdc.description.abstractThe Na +/K + pump is a nearly ubiquitous membrane protein in animal cells that uses the free energy of ATP hydrolysis to alternatively export 3Na + from the cell and import 2K + per cycle. This exchange of ions produces a steady-state outwardly directed current, which is proportional in magnitude to the turnover rate. Under certain ionic conditions, a sudden voltage jump generates temporally distinct transient currents mediated by the Na +/K + pump that represent the kinetics of extracellular Na + binding/release and Na + occlusion/deocclusion transitions. For many years, these events have escaped a proper thermodynamic treatment due to the relatively small electrical signal. Here, taking the advantages offered by the large diameter of the axons from the squid Dosidicus gigas, we have been able to separate the kinetic components of the transient currents in an extended temperature range and thus characterize the energetic landscape of the pump cycle and those transitions associated wit
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.uri
Sourcedc.sourceProceedings of the National Academy of Sciences of the United States of America
Keywordsdc.subjectP-type ATPases
Keywordsdc.subjectPump currents
Títulodc.titleEnergy landscape of the reactions governing the Na + deeply occluded state of the Na +/K +-ATPase in the giant axon of the Humboldt squid
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación SCOPUS

Files in this item


This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile