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In vivo analysis of the calcium signature in the plant Golgi apparatus reveals unique dynamics
Autor | dc.contributor.author | Ordenes, Viviana R. | |
Autor | dc.contributor.author | Moreno, Ignacio | |
Autor | dc.contributor.author | Maturana, Daniel | |
Autor | dc.contributor.author | Norambuena Morales, Lorena | |
Autor | dc.contributor.author | Trewavas, Anthony J. | |
Autor | dc.contributor.author | Orellana, Ariel | |
Fecha ingreso | dc.date.accessioned | 2019-03-15T16:03:15Z | |
Fecha disponible | dc.date.available | 2019-03-15T16:03:15Z | |
Fecha de publicación | dc.date.issued | 2012 | |
Cita de ítem | dc.identifier.citation | Cell Calcium, Volumen 52, Issue 5, 2018, Pages 397-404 | |
Identificador | dc.identifier.issn | 15321991 | |
Identificador | dc.identifier.issn | 01434160 | |
Identificador | dc.identifier.other | 10.1016/j.ceca.2012.06.008 | |
Identificador | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/165809 | |
Resumen | dc.description.abstract | The Golgi apparatus is thought to play a role in calcium homeostasis in plant cells. However, the calcium dynamics in this organelle is unknown in plants. To monitor the [Ca2+]Golgi in vivo, we obtained and analyzed Arabidopsis thaliana plants that express aequorin in the Golgi. Our results show that free [Ca2+] levels in the Golgi are higher than in the cytosol (0.70μM vs. 0.05μM, respectively). Stimuli such as cold shock, mechanical stimulation and hyperosmotic stress, led to a transient increase in cytosolic calcium; however, no instant change in the [Ca2+]Golgi concentration was detected. Nevertheless, a delayed increase in the [Ca2+]Golgi up to 2-3μM was observed. Cyclopiazonic acid and thapsigargin inhibited the stimuli-induced [Ca2+]Golgi increase, suggesting that [Ca2+]Golgi levels are dependent upon the activity of Ca2+-ATPases. Treatment of these plants with the synthetic auxin analog, 2,4-dichlorophenoxy acetic acid (2,4-D), produced a slow decrease of free calcium in the or | |
Idioma | dc.language.iso | en | |
Publicador | dc.publisher | Elsevier Ltd | |
Tipo de licencia | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
Link a Licencia | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
Fuente | dc.source | Cell Calcium | |
Palabras claves | dc.subject | Aequorin | |
Palabras claves | dc.subject | Auxin | |
Palabras claves | dc.subject | Calcium | |
Palabras claves | dc.subject | Golgi apparatus | |
Palabras claves | dc.subject | Plant | |
Título | dc.title | In vivo analysis of the calcium signature in the plant Golgi apparatus reveals unique dynamics | |
Tipo de documento | dc.type | Artículo de revista | |
Catalogador | uchile.catalogador | SCOPUS | |
Indización | uchile.index | Artículo de publicación SCOPUS | |
uchile.cosecha | uchile.cosecha | SI |
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