Mostrar el registro sencillo del ítem

Autordc.contributor.authorOrdenes, Viviana R. 
Autordc.contributor.authorMoreno, Ignacio 
Autordc.contributor.authorMaturana, Daniel 
Autordc.contributor.authorNorambuena Morales, Lorena 
Autordc.contributor.authorTrewavas, Anthony J. 
Autordc.contributor.authorOrellana, Ariel 
Fecha ingresodc.date.accessioned2019-03-15T16:03:15Z
Fecha disponibledc.date.available2019-03-15T16:03:15Z
Fecha de publicacióndc.date.issued2012
Cita de ítemdc.identifier.citationCell Calcium, Volumen 52, Issue 5, 2018, Pages 397-404
Identificadordc.identifier.issn15321991
Identificadordc.identifier.issn01434160
Identificadordc.identifier.other10.1016/j.ceca.2012.06.008
Identificadordc.identifier.urihttps://repositorio.uchile.cl/handle/2250/165809
Resumendc.description.abstractThe Golgi apparatus is thought to play a role in calcium homeostasis in plant cells. However, the calcium dynamics in this organelle is unknown in plants. To monitor the [Ca2+]Golgi in vivo, we obtained and analyzed Arabidopsis thaliana plants that express aequorin in the Golgi. Our results show that free [Ca2+] levels in the Golgi are higher than in the cytosol (0.70μM vs. 0.05μM, respectively). Stimuli such as cold shock, mechanical stimulation and hyperosmotic stress, led to a transient increase in cytosolic calcium; however, no instant change in the [Ca2+]Golgi concentration was detected. Nevertheless, a delayed increase in the [Ca2+]Golgi up to 2-3μM was observed. Cyclopiazonic acid and thapsigargin inhibited the stimuli-induced [Ca2+]Golgi increase, suggesting that [Ca2+]Golgi levels are dependent upon the activity of Ca2+-ATPases. Treatment of these plants with the synthetic auxin analog, 2,4-dichlorophenoxy acetic acid (2,4-D), produced a slow decrease of free calcium in the or
Idiomadc.language.isoen
Publicadordc.publisherElsevier Ltd
Tipo de licenciadc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link a Licenciadc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Fuentedc.sourceCell Calcium
Palabras clavesdc.subjectAequorin
Palabras clavesdc.subjectAuxin
Palabras clavesdc.subjectCalcium
Palabras clavesdc.subjectGolgi apparatus
Palabras clavesdc.subjectPlant
Títulodc.titleIn vivo analysis of the calcium signature in the plant Golgi apparatus reveals unique dynamics
Tipo de documentodc.typeArtículo de revista
Catalogadoruchile.catalogadorSCOPUS
Indizaciónuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


Descargar archivo

Icon

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivs 3.0 Chile
Excepto si se señala otra cosa, la licencia del ítem se describe como Attribution-NonCommercial-NoDerivs 3.0 Chile