Identification of cell surface-exposed proteins involved in the fimbria-mediated adherence of enteroaggregative Escherichia coli to intestinal cells
Author
dc.contributor.author
Izquierdo, Mariana
Author
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Navarro-Garcia, Fernando
Author
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Nava-Acosta, Raul
Author
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Nataro, James P.
Author
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Ruiz-Perez, Fernando
Author
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Farfan, Mauricio J.
Admission date
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2019-03-15T16:06:06Z
Available date
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2019-03-15T16:06:06Z
Publication date
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2014
Cita de ítem
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Infection and Immunity, Volumen 82, Issue 4, 2018, Pages 1719-1724
Identifier
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10985522
Identifier
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00199567
Identifier
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10.1128/IAI.01651-13
Identifier
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https://repositorio.uchile.cl/handle/2250/166115
Abstract
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Fimbria-mediated adherence to the intestinal epithelia is a key step in enteroaggregative Escherichia coli (EAEC) pathogenesis. To date, four fimbriae have been described for EAEC; aggregative adherence fimbria II (AAF/II) is the most important adherence factor for EAEC prototype strain 042. Previously, we described results showing that extracellular matrix (ECM) components might be involved in the recognition of AAF/II fimbriae by intestinal cells. In this study, we sought to identify novel potential receptors on intestinal epithelial cells recognized by the AAF/II fimbriae. Purified AafA-dsc protein, the major subunit of AAF/II fimbriae, was incubated with a monolayer of T84 cells, cross-linked to the surface-exposed T84 cell proteins, and immunoprecipitated by using anti-AafA antibodies. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of cellular proteins bound to AafA-dsc protein identified laminin (previously recognized as a potential receptor for AAF/II) and cy