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Authordc.contributor.authorBustamante, Mario 
Authordc.contributor.authorFernández Verdejo, Rodrigo 
Authordc.contributor.authorJaimovich Pérez, Enrique 
Authordc.contributor.authorBuvinic, Sonja 
Cita de ítemdc.identifier.citationAmerican Journal of Physiology - Endocrinology and Metabolism, Volumen 306, Issue 8, 2018,
Abstractdc.description.abstractInterleukin-6 (IL-6) is an important myokine that is highly expressed in skeletal muscle cells upon exercise. We assessed IL-6 expression in response to electrical stimulation (ES) or extracellular ATP as a known mediator of the excitation-transcription mechanism in skeletal muscle. We examined whether the canonical signaling cascade downstream of IL-6 (IL-6/JAK2/STAT3) also responds to muscle cell excitation, concluding that IL-6 influences its own expression through a positive loop. Either ES or exogenous ATP (100 μM) increased both IL-6 expression and p-STAT3 levels in rat myotubes, a process inhibited by 100 μM suramin and 2 U/ml apyrase. ATP also evoked IL-6 expression in both isolated skeletal fibers and extracts derived from whole FDB muscles. ATP increased IL-6 release up to 10-fold. STAT3 activation evoked by ATP was abolished by the JAK2 inhibitor HBC. Blockade of secreted IL-6 with a neutralizing antibody or preincubation with the STAT3 inhibitor VIII reduced STAT3 activatio
Publisherdc.publisherAmerican Physiological Society
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.uri
Sourcedc.sourceAmerican Journal of Physiology - Endocrinology and Metabolism
Keywordsdc.subjectMuscle plasticity
Keywordsdc.subjectPurinergic signaling
Keywordsdc.subjectSignal transducer and activator of transcription 3
Títulodc.titleElectrical stimulation induces IL-6 in skeletal muscle through extracellular ATP by activating Ca2+ signals and an IL-6 autocrine loop
Document typedc.typeArtículo de revista
dcterms.accessRightsdcterms.accessRightsAcceso Abierto
Indexationuchile.indexArtículo de publicación SCOPUS

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