GFP labelling and epigenetic enzyme expression of bone marrow-derived mesenchymal stem cells from bovine foetuses
Author
dc.contributor.author
Díaz, P.
Author
dc.contributor.author
Cuevas, F.
Author
dc.contributor.author
Peralta, O. A.
Admission date
dc.date.accessioned
2019-03-15T16:09:02Z
Available date
dc.date.available
2019-03-15T16:09:02Z
Publication date
dc.date.issued
2015
Cita de ítem
dc.identifier.citation
Research in Veterinary Science 99 (2015) 120–128
Identifier
dc.identifier.issn
15322661
Identifier
dc.identifier.issn
00345288
Identifier
dc.identifier.other
10.1016/j.rvsc.2014.12.019
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/166393
Abstract
dc.description.abstract
Mesenchymal stem cells (MSC) are multipotent progenitor cells defined by their ability to self-renew and give rise to differentiated progeny. Since MSC from adult tissues represent a promising source of cells for a wide range of cellular therapies, there is high scientific interest in better understanding the potential for genetic modification and the mechanism underlying differentiation. The main objective of this study was to evaluate the potential for gene delivery using a GFP vector and lipofectamine, and to quantify the expression of epigenetic enzymes during foetal bMSC multilineage differentiation. Proportion of GFP-positive cells achieved (15.7% +/- 3.5) indicated moderately low transfection efficiency. Analysis of DNA methyltransferase expression during MSC multilineage differentiation suggested no association with osteogenic and chondrogenic differentiation. However, up-regulation of KDM6B expression during osteogenic differentiation was associated with adoption of osteogenic lineage. Furthermore, increase in epigenetic enzyme expression suggested an intense epigenetic regulation during adipogenic differentiation.