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Author | dc.contributor.author | Molinos-Albert, Luis M. | |
Author | dc.contributor.author | Bilbao, Eneritz | |
Author | dc.contributor.author | Agulló, Luis | |
Author | dc.contributor.author | Marfil, Silvia | |
Author | dc.contributor.author | García, Elisabet | |
Author | dc.contributor.author | Concepción, Maria Luisa Rodríguez De La | |
Author | dc.contributor.author | Izquierdo-Useros, Nuria | |
Author | dc.contributor.author | Vilaplana, Cristina | |
Author | dc.contributor.author | Nieto-Garai, Jon A. | |
Author | dc.contributor.author | Contreras, F. Xabier | |
Author | dc.contributor.author | Floor, Martin | |
Author | dc.contributor.author | Cardona, Pere J. | |
Author | dc.contributor.author | Martinez-Picad | |
Admission date | dc.date.accessioned | 2019-03-18T11:55:32Z | |
Available date | dc.date.available | 2019-03-18T11:55:32Z | |
Publication date | dc.date.issued | 2017 | |
Cita de ítem | dc.identifier.citation | Scientific Reports, Volumen 7, | |
Identifier | dc.identifier.issn | 20452322 | |
Identifier | dc.identifier.other | 10.1038/srep40800 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/166985 | |
Abstract | dc.description.abstract | © The Author(s) 2017. The HIV-1 gp41 Membrane Proximal External Region (MPER) is recognized by broadly neutralizing antibodies and represents a promising vaccine target. However, MPER immunogenicity and antibody activity are influenced by membrane lipids. To evaluate lipid modulation of MPER immunogenicity, we generated a 1-Palmitoyl-2-oleoylphosphatidylcholine (POPC)-based proteoliposome collection containing combinations of phosphatidylserine (PS), GM3 ganglioside, cholesterol (CHOL), sphingomyelin (SM) and the TLR4 agonist monophosphoryl lipid A (MPLA). A recombinant gp41-derived miniprotein (gp41-MinTT) exposing the MPER and a tetanus toxoid (TT) peptide that favors MHC-II presentation, was successfully incorporated into lipid mixtures (>85%). Immunization of mice with soluble gp41-MinTT exclusively induced responses against the TT peptide, while POPC proteoliposomes generated potent anti-gp41 IgG responses using lower protein doses. The combined addition of PS and GM3 or CHOL/SM t | |
Lenguage | dc.language.iso | en | |
Publisher | dc.publisher | Nature Publishing Group | |
Type of license | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
Link to License | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
Source | dc.source | Scientific Reports | |
Keywords | dc.subject | Multidisciplinary | |
Título | dc.title | Proteoliposomal formulations of an HIV-1 gp41-based miniprotein elicit a lipid-dependent immunodominant response overlapping the 2F5 binding motif | |
Document type | dc.type | Artículo de revista | |
dcterms.accessRights | dcterms.accessRights | Acceso Abierto | |
Cataloguer | uchile.catalogador | SCOPUS | |
Indexation | uchile.index | Artículo de publicación SCOPUS | |
uchile.cosecha | uchile.cosecha | SI | |
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