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Author dc.contributor.author Montes, Matías
Author dc.contributor.author Moreira Ramos, Sandra
Author dc.contributor.author Rojas, Diego A.
Author dc.contributor.author Urbina, Fabiola
Author dc.contributor.author Käufer, Norbert F.
Author dc.contributor.author Maldonado, Edio
Admission date dc.date.accessioned 2019-03-18T11:55:40Z
Available date dc.date.available 2019-03-18T11:55:40Z
Publication date dc.date.issued 2017
Cita de ítem dc.identifier.citation FEBS Journal, Volumen 284, Issue 4, 2018, Pages 615-633
Identifier dc.identifier.issn 17424658
Identifier dc.identifier.issn 1742464X
Identifier dc.identifier.other 10.1111/febs.14006
Identifier dc.identifier.uri https://repositorio.uchile.cl/handle/2250/167036
Abstract dc.description.abstract © 2017 Federation of European Biochemical Societies In Schizosaccharomyces pombe, ribosomal protein gene (RPG) promoters contain a TATA box analog, the HomolD box, which is bound by the Rrn7 protein. Despite the importance of ribosome biogenesis for cell survival, the mechanisms underlying RPG transcription remain unknown. In this study, we found that components of the RNA polymerase II (RNAPII) system, consisting of the initiation or general transcription factors (GTFs) TFIIA, IIB, IIE, TATA-binding protein (TBP) and the RNAPII holoenzyme, interacted directly with Rrn7 in vitro, and were able to form a preinitiation complex (PIC) on the HomolD box. PIC complex formation follows an ordered pathway on these promoters. The GTFs and RNAPII can also be cross-linked to HomolD-containing promoters in vivo. In an in vitro reconstituted transcription system, RNAPII components and Rrn7 were necessary for HomolD-directed transcription. The Mediator complex was required for basal transcription fr
Lenguage dc.language.iso en
Publisher dc.publisher Blackwell Publishing Ltd
Type of license dc.rights Attribution-NonCommercial-NoDerivs 3.0 Chile
Link to License dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Source dc.source FEBS Journal
Keywords dc.subject general transcription factor
Keywords dc.subject HomolD
Keywords dc.subject ribosomal protein
Keywords dc.subject RNA polymerase II
Keywords dc.subject Schizosaccharomyces pombe
Título dc.title RNA polymerase II components and Rrn7 form a preinitiation complex on the HomolD box to promote ribosomal protein gene expression in Schizosaccharomyces pombe
Document type dc.type Artículo de revista
Cataloguer uchile.catalogador SCOPUS
Indexation uchile.index Artículo de publicación SCOPUS
uchile.cosecha uchile.cosecha SI
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