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Authordc.contributor.authorSagredo, Alfredo I.
Authordc.contributor.authorSagredo, Eduardo A.
Authordc.contributor.authorPola, Victor
Authordc.contributor.authorEcheverría, César
Authordc.contributor.authorAndaur, Rodrigo
Authordc.contributor.authorMichea Acevedo, Luis
Authordc.contributor.authorStutzin Schottlander, Andrés
Authordc.contributor.authorSimon, Felipe
Authordc.contributor.authorMarcelain Cubillos, Katherine
Authordc.contributor.authorArmisén, Ricardo
Admission datedc.date.accessioned2019-05-31T15:35:22Z
Available datedc.date.available2019-05-31T15:35:22Z
Publication datedc.date.issued2019
Cita de ítemdc.identifier.citationJ Cell Physiol. 2019;234:2037-2050
Identifierdc.identifier.issn10974652
Identifierdc.identifier.issn00219541
Identifierdc.identifier.other10.1002/jcp.27371
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/169731
Abstractdc.description.abstractTransient Receptor Potential Melastatin 4 (TRPM4) is a Ca2+‐activated and voltage‐ dependent monovalent cation channel, which depolarizes the plasma cell membrane, thereby modulating Ca2+ influx across Ca2+‐permeable pathways. TRPM4 is involved in different physiological processes such as T cell activation and the migration of endothelial and certain immune cells. Overexpression of this channel has been reported in various types of tumors including prostate cancer. In this study, a significant overexpression of TRPM4 was found only in samples from cancer with a Gleason score higher than 7, which are more likely to spread. To evaluate whether TRPM4 overexpression was related to the spreading capability of tumors, TRPM4 was knockdown by using shRNAs in PC3 prostate cancer cells and the effect on cellular migration and invasion was analyzed. PC3 cells with reduced levels of TRPM4 (shTRPM4) display a decrease of the migration/invasion capability. A reduction in the expression of Snail1, a canonical epithelial to mesenchymal transition (EMT) transcription factor, was also observed. Consistently, these cells showed a significant change in the expression of key EMT markers such as MMP9, E‐cadherin/N‐cadherin, and vimentin, indicating a partial reversion of the EMT process. Whereas, the overexpression of TRPM4 in LnCaP cells resulted in increased levels of Snail1, reduction in the expression of E‐cadherin and increase in their migration potential. This study suggests a new and indirect mechanism of regulation of migration/invasion process by TRPM4 in prostate cancer cells, by inducing the expression of Snail1 gene and consequently, increasing the EMT.
Lenguagedc.language.isoen
Publisherdc.publisherWiley
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceJournal of Cellular Physiology
Keywordsdc.subjectEMT
Keywordsdc.subjectInvasion
Keywordsdc.subjectProstate cancer
Keywordsdc.subjectSnail1
Keywordsdc.subjectTRPM4
Títulodc.titleTRPM4 channel is involved in regulating epithelial to mesenchymal transition, migration, and invasion of prostate cancer cell lines
Document typedc.typeArtículo de revista
dcterms.accessRightsdcterms.accessRightsAcceso abierto
Catalogueruchile.catalogadorlaj
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile