TGF-β activity in acid bone lysate adsorbs to titanium surface
Author
dc.contributor.author
Strauss Avendaño, Franz
Author
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Di Summa, Francesca
Author
dc.contributor.author
Stähli, Alexandra
Author
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Matos, Luiza
Author
dc.contributor.author
Vaca, Fabiola
Author
dc.contributor.author
Schuldt, Guenther
Author
dc.contributor.author
Gruber, Reinhard
Admission date
dc.date.accessioned
2019-10-14T15:41:05Z
Available date
dc.date.available
2019-10-14T15:41:05Z
Publication date
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2019
Cita de ítem
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Clinical Implant Dentistry and Related Research, Volumen 21, Issue 2, 2019, Pages 336-343
Identifier
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17088208
Identifier
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15230899
Identifier
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10.1111/cid.12734
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/171533
Abstract
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Objectives: Osteoblasts lay down new bone on implant surfaces. The underlying cellular mechanism and the spatio-temporal mode of action, however, remain unclear. It can be proposed that growth factors released upon acidification by osteoclasts adsorb to the implant surface and control the early stages of osseointegration. Methods: To simulate bone lysis by osteoclasts, titanium discs were exposed to acid bone lysate (ABL) followed by vigorous washing and seeding of oral fibroblasts. The expression of TGF-β target genes interleukin 11 (IL11) and NADPH oxidase 4 (NOX4) was evaluated by reverse transcriptase polymerase chain reaction and IL11 ELISA. TGF-β signaling activation was assessed via Smad2/3 immunofluorescence. The impact of ABL on osteogenic differentiation was determined with murine ST2 mesenchymal stromal cells. Results: We report here that ABL-conditioned titanium discs, i