Immunization of mice with chimeric antigens displaying selected epitopes confers protection against intestinal colonization and renal damage caused by Shiga toxin-producing Escherichia coli
Author
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Montero Forero, David
Author
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Canto Fuentes, Felipe del
Author
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Salazar Garrido, Juan Carlos
Author
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Céspedes Ulloa, Sandra
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Cádiz Núñez, Leandro
Author
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Arenas Salinas, Mauricio
Author
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Reyes, José
Author
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Oñate, Ángel
Author
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Vidal Álvarez, Roberto
Admission date
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2020-05-06T19:49:50Z
Available date
dc.date.available
2020-05-06T19:49:50Z
Publication date
dc.date.issued
2020
Cita de ítem
dc.identifier.citation
npj Vaccines (2020) 20
es_ES
Identifier
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10.1038/s41541-020-0168-7
Identifier
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https://repositorio.uchile.cl/handle/2250/174460
Abstract
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Shiga toxin-producing Escherichia coli (STEC) cause diarrhea and dysentery, which may progress to hemolytic uremic syndrome (HUS). Vaccination has been proposed as a preventive approach against STEC infection; however, there is no vaccine for humans and those used in animals reduce but do not eliminate the intestinal colonization of STEC. The OmpT, Cah and Hes proteins are widely distributed among clinical STEC strains and are recognized by serum IgG and IgA in patients with HUS. Here, we develop a vaccine formulation based on two chimeric antigens containing epitopes of OmpT, Cah and Hes proteins against STEC strains. Intramuscular and intranasal immunization of mice with these chimeric antigens elicited systemic and local long-lasting humoral responses. However, the class of antibodies generated was dependent on the adjuvant and the route of administration. Moreover, while intramuscular immunization with the combination of the chimeric antigens conferred protection against colonization by STEC O157:H7, the intranasal conferred protection against renal damage caused by STEC O91:H21. This preclinical study supports the potential use of this formulation based on recombinant chimeric proteins as a preventive strategy against STEC infections.
es_ES
Patrocinador
dc.description.sponsorship
FONDEF
ID16I10140
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT
1161161
Immunization of mice with chimeric antigens displaying selected epitopes confers protection against intestinal colonization and renal damage caused by Shiga toxin-producing Escherichia coli