Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide
Author
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Riveros Salvatierra, Ana
Author
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Eggeling, Cynthia
Author
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Riquelme Barrios, Sebastián
Author
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Adura, Carolina
Author
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López Iglesias, Carmen
Author
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Guzmán, Fanny
Author
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Araya Fuentes, Eyleen
Author
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Almada, Mario
Author
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Juárez, Josue
Author
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Valdez, Miguel A.
Author
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Fuentevilla Morgado, Ignacio
Author
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López, Olga
Author
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Kogan Bocian, Marcelo
Admission date
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2020-05-06T19:52:29Z
Available date
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2020-05-06T19:52:29Z
Publication date
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2020
Cita de ítem
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International Journal of Nanomedicine 2020:15 1837–1851
es_ES
Identifier
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10.2147/IJN.S237820
Identifier
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https://repositorio.uchile.cl/handle/2250/174461
Abstract
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Introduction: Gold nanorods are highly reactive, have a large surface-to-volume ratio, and can be functionalized with biomolecules. Gold nanorods can absorb infrared electromagnetic radiation, which is subsequently dispersed as local heat. Gold nanoparticles can be used as powerful tools for the diagnosis and therapy of different diseases. To improve the biological barrier permeation of nanoparticles with low cytotoxicity, in this study, we conjugated gold nanorods with cell-penetrating peptides (oligoarginines) and with the amphipathic peptide CLPFFD.
Methods: We studied the interaction of the functionalized gold nanorods with biological membrane models (liposomes) by dynamic light scattering, transmission electron microscopy and the Langmuir balance. Furthermore, we evaluated the effects on cell viability and permeability with an MTS assay and TEM.
Results and Discussion: The interaction study by DLS, the Langmuir balance and cryoTEM support that GNR-Arg7CLPFFD enhances the interactions between GNRs and biological membranes. In addition, cells treated with GNR-Arg7CLPFFD internalized 80% more nanoparticles than cells treated with GNR alone and did not induce cell damage.
Conclusion: Our results indicate that incorporation of an amphipathic sequence into oligoarginines for the functionalization of gold nanorods enhances biological membrane nanoparticle interactions and nanoparticle cell permeability with respect to nanorods functionalized with oligoarginine. Overall, functionalized gold nanorods with amphipathic arginine rich peptides might be candidates for improving drug delivery by facilitating biological barrier permeation.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT
3130654
1170929
1190623
FONDAP
15130011
Nucleo UNAB
36/18N
CONACYT/CONICYT (Mexico)
204393
CONICYT/CONACYT (Chile)
Fondequip
EQM170111