Downsizing class II lasso peptides: genome mining-guided isolation of huascopeptin containing the first Gly1-Asp7 macrocycle
Author
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Cortés Albayay, Carlos
Author
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Jarmusch, Scott A.
Author
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Trusch, Franziska
Author
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Ebel, Rainer
Author
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Andrews Farrow, Bárbara
Author
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Jaspars, Marcel
Author
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Asenjo, Juan
Admission date
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2020-05-06T20:33:31Z
Available date
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2020-05-06T20:33:31Z
Publication date
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2020
Cita de ítem
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J. Org. Chem. 2020, 85, 1661−1667
es_ES
Identifier
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10.1021/acs.joc.9b02231
Identifier
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https://repositorio.uchile.cl/handle/2250/174474
Abstract
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A new lasso peptide, huascopeptin, was isolated following genome-mined discovery of a new biosynthetic gene cluster in extremotolerant Streptomyces huasconensis HST28(T) from Salar de Huasco, Atacama Desert, Chile. Compound 1 is a 13-residue class II lasso peptide containing a novel Gly1-Asp7 macrolactam ring, a three-residue loop, and a three-residue tail, making it the smallest lasso peptide isolated to date. The lasso structure was confirmed using NOE restraint-based molecular dynamics simulations.
es_ES
Patrocinador
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Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT): PFCHA/DOCTORADO BECAS CHILE/2016-21160585
CONICYT Basal Centre Grant for the Centre for Biotechnology and Bioengineering, CeBiB: FB0001
University of Aberdeen