SRL pathogenicity island contributes to the metabolism of D-aspartate via an aspartate racemase in Shigella flexneri YSH6000
Author
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Henríquez, Tania
Author
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Salazar Garrido, Juan
Author
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Marvasi, Massimiliano
Author
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Shah, Ajit
Author
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Corsini, Gino
Author
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Toro Ugalde, Cecilia
Admission date
dc.date.accessioned
2020-07-02T15:10:51Z
Available date
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2020-07-02T15:10:51Z
Publication date
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2020
Cita de ítem
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PLoS ONE 15(1): e0228178 Jan 2020
es_ES
Identifier
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10.1371/journal.pone.0228178
Identifier
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https://repositorio.uchile.cl/handle/2250/175754
Abstract
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In recent years, multidrug resistance of Shigella strains associated with genetic elements like pathogenicity islands, have become a public health problem. The Shigella resistance locus pathogenicity island (SRL PAI) of S. flexneri 2a harbors a 16Kbp region that contributes to the multidrug resistance phenotype. However, there is not much information about other functions such as metabolic, physiologic or ecological ones. For that, wild type S. flexneri YSH6000 strain, and its spontaneous SRL PAI mutant, 1363, were used to study the contribution of the island in different growth conditions. Interestingly, when both strains were compared by the Phenotype Microarrays, the ability to metabolize D-aspartic acid as a carbon source was detected in the wild type strain but not in the mutant. When D-aspartate was added to minimal medium with other carbon sources such as mannose or mannitol, the SRL PAI-positive strain was able to metabolize it, while the SRL PAI-negative strain did not. In order to identify the genetic elements responsible for this phenotype, a bioinformatic analysis was performed and two genes belonging to SRL PAI were found: orf8, coding for a putative aspartate racemase, and orf9, coding for a transporter. Thus, it was possible to measure, by an indirect analysis of racemization activity in minimal medium supplemented only with D-aspartate, that YSH6000 strain was able to transform the D-form into L-, while the mutant was impaired to do it. When the orf8-orf9 region from SRL island was transformed into S. flexneri and S. sonnei SRL PAI-negative strains, the phenotype was restored. Although, when single genes were cloned into plasmids, no complementation was observed. Our results strongly suggest that the aspartate racemase and the transporter encoded in the SRL pathogenicity island are important for bacterial survival in environments rich in D-aspartate.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT
1130394
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
8994
Uch1304
Middlesex University London
BioMentoring Program, Faculty of Biology, Ludwig-Maximilians Universitat Munchen, Germany 2018-2019