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Authordc.contributor.authorLemaitre, Dominique 
Authordc.contributor.authorLlavero Hurtado, Maica 
Authordc.contributor.authorDe Gregorio, Cristian 
Authordc.contributor.authorOñate, Maritza G. 
Authordc.contributor.authorMartínez Bravo, Gabriela 
Authordc.contributor.authorCatenaccio, Alejandra 
Authordc.contributor.authorWishart, Thomas M. 
Authordc.contributor.authorCourt, Felipe A. 
Cita de ítemdc.identifier.citationMolecular Neurobiology 57(1): 4232-4249 (2020)es_ES
Abstractdc.description.abstractPeripheral nerve injuries result in motor and sensory dysfunction which can be recovered by compensatory or regenerative processes. In situations where axonal regeneration of injured neurons is hampered, compensation by collateral sprouting from uninjured neurons contributes to target reinnervation and functional recovery. Interestingly, this process of collateral sprouting from uninjured neurons has been associated with the activation of growth-associated programs triggered by Wallerian degeneration. Nevertheless, themolecular alterations at the transcriptomic level associated with these compensatory growthmechanisms remain to be fully elucidated. We generated a surgical model of partial sciatic nerve injury in mice to mechanistically study degenerationinduced collateral sprouting from spared fibers in the peripheral nervous system. Using next-generation sequencing and Ingenuity Pathway Analysis, we described the sprouting-associated transcriptome of uninjured sensory neurons and compare it with the activated by regenerating neurons. In vitro approacheswere used to functionally assess sprouting gene candidates in the mechanisms of axonal growth. Using a novel animal model, we provide the first description of the sprouting transcriptome observed in uninjured sensory neurons after nerve injury. This collateral sprouting-associated transcriptome differs from that seen in regenerating neurons, suggesting a molecular program distinct from axonal growth.We further demonstrate that genetic upregulation of novel sproutingassociated genes activates a specific growth program in vitro, leading to increased neuronal branching. These results contribute to our understanding of the molecular mechanisms associated with collateral sprouting in vivo. The data provided here will therefore be instrumental in developing therapeutic strategies aimed at promoting functional recovery after injury to the nervous system.es_ES
Patrocinadordc.description.sponsorshipGeroscience Center for Brain Health and Metabolism FONDAP-15150012 Takeda Pharmaceutical Company Ltd P09-015-F Formation of Advance Human Capital Program of CONICYT 21110017 ISPG from the BBSRC FONDECYT-1150766 FONDECYT1190518es_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Sourcedc.sourceMolecular Neurobiologyes_ES
Keywordsdc.subjectAxonal regenerationes_ES
Keywordsdc.subjectCollateral sproutinges_ES
Keywordsdc.subjectNerve injuryes_ES
Keywordsdc.subjectSciatic nervees_ES
Títulodc.titleCollateral Sprouting of Peripheral Sensory Neurons Exhibits a Unique Transcriptomic Profilees_ES
Document typedc.typeArtículo de revistaes_ES
dcterms.accessRightsdcterms.accessRightsAcceso Abierto
Indexationuchile.indexArtículo de publicación ISI
Indexationuchile.indexArtículo de publicación SCOPUS

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile