Impact of DBBM fragments on the porosity of the calvarial bone: A pilot study on mice
Author
dc.contributor.author
Kuchler, Ulrike
Author
dc.contributor.author
Heimel, Patrick
Author
dc.contributor.author
Staehli, Alexandra
Author
dc.contributor.author
Strauss, Franz Josef
Author
dc.contributor.author
Luza, Bernadette
Author
dc.contributor.author
Gruber, Reinhard
Admission date
dc.date.accessioned
2021-04-29T21:08:09Z
Available date
dc.date.available
2021-04-29T21:08:09Z
Publication date
dc.date.issued
2020
Cita de ítem
dc.identifier.citation
Materials 2020, 13, 4748
es_ES
Identifier
dc.identifier.other
10.3390/ma13214748
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/179354
Abstract
dc.description.abstract
Deproteinized bovine bone mineral (DBBM) is brittle and can break into fragments. Here, we examined whether DBBM fragments have an impact on mice calvarial bone during bone augmentation. DBBM was either randomly crushed (DBBM fragments) or left undisturbed (DBBM granules). Then, DBBM fragments or original DBBM granules were placed onto calvarial bone in 20 BALB/c mice. Following random allocation, ten mice received DBBM fragments and ten mice received original DBBM granules. After fourteen days of healing, micro computed tomography (micro-CT) and histological analysis of the augmented sites were performed. The primary outcome was the porosity of the calvarial bone. The micro-CT analysis revealed that DBBM fragments failed to significantly change the porosity of the calvarial bone as compared with original DBBM granules, despite the slightly higher bone resorption in the DBBM fragment group, 10.3% (CI 6.3-11.6) versus 6.1% (CI 4.1-7.8, p = 0.355), respectively. The cortical bone volume was not altered by DBBM fragments as compared with original DBBM granules, i.e., 79.0% (CI 78.9-81.2) versus 81.5% (CI 80.1-83.3, p = 0.357), respectively. The DBBM fragment group revealed similar bone thickness values as compared with the DBBM granules group, i.e., 0.26 mm (CI 0.23-0.29) versus 0.25 mm (CI 0.22-0.27, p = 0.641), respectively. The histological evaluation supported the micro-CT observations, displaying minor signs of porosity and resorption. The particle-size distribution analysis confirmed a shift towards smaller particle sizes in the DBBM fragment group. These findings suggest that DBBM fragments behave similarly to original DBBM granules in terms of bone morphological changes at augmented sites.
es_ES
Patrocinador
dc.description.sponsorship
Osteology Foundation, Switzerland
14-126
Osteology Foundation
17-219
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)