MMP-8, TRAP-5, and OPG levels in GCF diagnostic potential to discriminate between healthy patients’, mild and severe periodontitis sites
Author
dc.contributor.author
Hernández, Marcela
Author
dc.contributor.author
Baeza Paredes, Mauricio
Author
dc.contributor.author
Contreras Balbontín, Johanna
Author
dc.contributor.author
Sorsa, Timo
Author
dc.contributor.author
Tervahartiala, Taina
Author
dc.contributor.author
Valdés Salgado, Macarena
Author
dc.contributor.author
Chaparro, Alejandra
Author
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Hernández Ríos, Patricia
Admission date
dc.date.accessioned
2021-05-19T19:14:50Z
Available date
dc.date.available
2021-05-19T19:14:50Z
Publication date
dc.date.issued
2020
Cita de ítem
dc.identifier.citation
Biomolecules 2020, 10, 1500
es_ES
Identifier
dc.identifier.other
10.3390/biom10111500
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/179686
Abstract
dc.description.abstract
Biomarkers represent promising aids in periodontitis, host-mediate diseases of the tooth-supporting tissues. We assessed the diagnostic potential of matrix metalloproteinase-8 (MMP-8), tartrate-resistant acid phosphatase-5 (TRAP-5), and osteoprotegerin (OPG) to discriminate between healthy patients', mild and severe periodontitis sites. Thirty-one otherwise healthy volunteers with and without periodontal disease were enrolled at the Faculty of Dentistry, University of Chile. Periodontal parameters were examined and gingival crevicular fluid was sampled from mild periodontitis sites (M; n = 42), severe periodontitis sites (S; n = 59), and healthy volunteer sites (H; n = 30). TRAP-5 and OPG were determined by commercial multiplex assay and MMP-8 by the immunofluorometric (IFMA) method. STATA software was used. All biomarkers showed a good discrimination performance. MMP-8 had the overall best performance in regression models and Receiver Operating Characteristic (ROC) curves, with high discrimination of healthy from periodontitis sites (area under the curve (AUC) = 0.901). OPG showed a very high diagnostic precision (AUC >= 0.95) to identify severe periodontitis sites (S versus H + M), while TRAP-5 identified both healthy and severe sites. As conclusions, MMP-8, TRAP-5, and OPG present a high precision potential in the identification of periodontal disease destruction, with MMP-8 as the most accurate diagnostic biomarker.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT
1090741
1090046
1200098
University of Helsinki Research Foundation, Helsinki, Finland
TYH2016251
TYH2017251
TYH2018229
TYH2019319
Y1014SL017
Y1014SL018
Y1014SULE1
Finnish Dental Society Apollonia
Karolinska Institutet, Stockholm, Sweden