Acylglycerol synthesis including EPA and DHA from rainbow trout (Oncorhynchus mykiss) belly flap oil and caprylic acid catalyzed by Thermomyces lanuginosus lipase under supercritical carbon dioxide
Author
dc.contributor.author
Pando, M. Elsa
Author
dc.contributor.author
Rodríguez Melis, Alicia
Author
dc.contributor.author
Valenzuela Pedevila, María Antonieta
Author
dc.contributor.author
Berríos, Macarena
Author
dc.contributor.author
Rivera, Matías
Author
dc.contributor.author
Romero Palacios, Nalda
Author
dc.contributor.author
Barriga, Andrés
Author
dc.contributor.author
Aubourg, Santiago
Admission date
dc.date.accessioned
2021-05-24T23:25:47Z
Available date
dc.date.available
2021-05-24T23:25:47Z
Publication date
dc.date.issued
2021
Cita de ítem
dc.identifier.citation
European Food Research and Technology (2021) 247:499–511
es_ES
Identifier
dc.identifier.other
10.1007/s00217-020-03643-4
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/179777
Abstract
dc.description.abstract
Supercritical carbon dioxide (SCCO2) was studied as a medium for the esterification of eicosapentaenoic acid (n-3 C20:5, EPA) or docosahexaenoic acid (n-3 C22:6, DHA) and caprylic acid (C8:0, CA) in structured triacylglycerols (sTAG) using Thermomyces lanuginosus lipase as biocatalyst. Process variables (n-3 long-chain polyunsaturated fatty acid: CA, n-3 LCPUFA:CA content ratio), glycerol content (wt. %), and supercritical time, temperature and pressure were optimized by the Response Surface Methodology through a central composite design of 2(5-1) + star. Synthesis of sTAG with EPA, DHA and CA under SCCO2 was significantly affected by the n-3 LCPUFA:CA content ratio and supercritical time. MALDI-TOF mass spectrometry revealed that acylglycerols with the highest levels of EPA or DHA content in the sn-2 position were obtained when the following variables conditions were applied: 50% (n-3 LCPUFA:CA content ratio), 40 degrees C (supercritical temperature), 20 MPa (supercritical pressure), 4 h (supercritical time) and 20.0 wt. % (glycerol concentration). For such experimental conditions, esterification catalyzed by Thermomyces lanuginosus lipase under supercritical carbon dioxide allowed obtaining sTAG synthesized with 54.95% of CA, 11.64% of EPA and 13.77% of DHA.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica, FONDECYT program (Government of Chile)
1120627
Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santiago, Chile
Acylglycerol synthesis including EPA and DHA from rainbow trout (Oncorhynchus mykiss) belly flap oil and caprylic acid catalyzed by Thermomyces lanuginosus lipase under supercritical carbon dioxide