Chemical profiling, antioxidant, anticholinesterase, and antiprotozoal potentials of artemisia copa phil. (Asteraceae)
Author
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Larrazábal Fuentes, María José
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Fernández Galleguillos, Carlos
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Palma Ramírez, Jenifer
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Romero Parra, Javier Hernán
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Sepúlveda, Kevin
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Galetovic, Alexandra
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González, Jorge
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Paredes, Adrián
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Bórquez, Jorge
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Simirgiotis, Mario J.
Author
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Echeverría, Javier
Admission date
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2021-06-09T19:35:02Z
Available date
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2021-06-09T19:35:02Z
Publication date
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2020
Cita de ítem
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Frontiers in Pharmacology December 2020 | Volume 11 | Article 594174
es_ES
Identifier
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10.3389/fphar.2020.594174
Identifier
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https://repositorio.uchile.cl/handle/2250/180062
Abstract
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Artemisia copa Phil. (Asteraceae) (known as copa-copa) is a native species of Chile used as an infusion in traditional medicine by Atacamenos people in the Altiplano, highlands of northern Chile. In this research, we have investigated for the first time the cholinesterase inhibition potential against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), and the chemical profiling of the infusions prepared from the aerial parts of A. copa by high resolution spectrometry. In addition, total phenolic, total flavonoid content, antioxidant (DPPH, FRAP, and ORAC) and antiprozoal activity were tested. Artemisia copa showed good inhibitory activity against AChE and BChE (3.92 +/- 0.08 mu g/ml and 44.13 +/- 0.10 mu g/ml). The infusion displayed a total phenolics content of 155.6 +/- 2.9 mg of gallic acid equivalents/g and total flavonoid content of 5.5 +/- 0.2 mg quercetin equivalents/g. Additionally, trypanocidal activity against Trypanosoma cruzi was found (LD50 of 131.8 mu g/ml). Forty-seven metabolites were detected in the infusion of A. copa including several phenolic acids and flavonoids which were rapidly identified using ultrahigh performance liquid chromatography orbitrap mass spectrometry analysis (UHPLC-Orbitrap-MS) for chemical profiling. The major compounds identified in the infusions were studied by molecular docking against AChE and BChE. The UHPLC-MS fingerprints generated can be also used for the authentication of these endemic species. These findings reveal that A. copa infusions can be used as beverages with protective effects.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT
1180059
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
PAI/ACADEMIA 79160109
MINEDUC-UA project
ANT 1856
3190794