Anti-quorum sensing and anti-biofilm activity of nickel oxide nanoparticles against Pseudomonas aeruginosa
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2020Metadata
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Maruthupandy, Muthuchamy
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Anti-quorum sensing and anti-biofilm activity of nickel oxide nanoparticles against Pseudomonas aeruginosa
Abstract
Nickel oxide nanoparticles (NiO NPs) were prepared by a chemical reduction method. The physical and morphological observations of NiO NPs were assessed by powder X-ray diffraction, thermogravimetric analysis, Fourier transform infrared spectroscopy, and UV-vis diffuse reflectance spectroscopy, scanning electron microscopy and transmission electron microscopy. Furthermore, the in vitro inhibition studies of violacein degradation in Chromobacterium violaceum ATCC 12472 and biofilm eradication in P. aeruginosa were detected at 50 mu g/mL and 60 mu g/mL, respectively. In addition, the biofilm inhibition was confirmed by ring biofilm degradation and time-kill variation assays. The live/dead cell variations, biofilm architecture damage and cell wall modifications of NiO NPs were investigated using confocal laser scanning microscope and SEM. In addition, NiO NPs were found to act as anti-biofilm agent against P. aeruginosa. Finally the in-vivo toxicity of NiO NPs was investigated against Artemia franciscana, suggesting low toxicity.
Patrocinador
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT
3180128
National Natural Science Foundation of China (NSFC)
41950410573
91951205
31670009
China Postdoctoral Science Foundation
2019M663213
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Artículo de publicación ISI Artículo de publicación SCOPUS
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Journal of Environmental Chemical Engineering 8 (2020) 104533
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