Electrocatalytic determination of NADH by means of electrodes modified with MWCNTs and nitroaromatic compounds
Author
dc.contributor.author
Contreras, G.
Author
dc.contributor.author
Barrientos, C
Author
dc.contributor.author
Moscoso, R.
Author
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Álvarez Lueje, Alejandro
Author
dc.contributor.author
Squella Serrano, Juan
Admission date
dc.date.accessioned
2021-06-09T21:51:13Z
Available date
dc.date.available
2021-06-09T21:51:13Z
Publication date
dc.date.issued
2020
Cita de ítem
dc.identifier.citation
Microchemical Journal 159 (2020) 105422
es_ES
Identifier
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10.1016/j.microc.2020.105422
Identifier
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https://repositorio.uchile.cl/handle/2250/180082
Abstract
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In this work, we have both synthesized and studied 4-phenylbutyl-3,5-dinitrobenzoate (3,5-DNBPh) as a potential precursor of an electrocatalytic mediator for the oxidation of NADH, with the di-nitro compound immobilized in a three-dimensional network of glassy carbon electrodes modified with MWCNTs. This di-nitro compound is activated electrochemically to selectively generate one nitroso-hydroxylamine redox pair, which ultimately acts as a redox mediator of NADH oxidation. Thus, NADH electrocatalysis displaces the oxidation potential of NADH with the formation of a single voltammetric peak, the displacement occurs from Ep = +0.374 V in GCE/MWCNT to +0.001 V in GCE/MWCNT/3,5-DNBPh (very close to 0 V, Delta Ep = 0.373 V). The 4-phenylbutyl-3,5-dinitrobenzoate mediator is sensitive to NADH concentration, and a linear regression between 0.1 and 0.6 mM NADH (r(2) = 0.99) was obtained, with a detection limit of 22.3 mu M (S/N = 3) and a quantification limit of 74.2 mu M (S/N = 10). The developed modified electrode presents low NADH electrocatalytic oxidation potentials, and an interesting and novel advantage is that the preparation of the electrodes is fast, clean and effective, allowing biosensors to be obtained in a few steps.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT
1170054
1210899