Systemic and Extraradicular Bacterial Translocation in Apical Periodontitis
Author
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Bordagaray San Martín, María José
Author
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Fernández, Alejandra
Author
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Garrido, Mauricio
Author
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Astorga, Jéssica
Author
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Hoare Teuche, Anilei Paz
Author
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Hernández Ríos, Marcela
Admission date
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2021-10-25T20:57:15Z
Available date
dc.date.available
2021-10-25T20:57:15Z
Publication date
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2021
Cita de ítem
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Frontiers in Cellular and Infection Microbiology March 2021 | Volume 11 | Article 649925
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Identifier
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10.3389/fcimb.2021.649925
Identifier
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https://repositorio.uchile.cl/handle/2250/182388
Abstract
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Apical periodontitis is an inflammatory disease of microbial etiology. It has been suggested that endodontic bacterial DNA might translocate to distant organs via blood vessels, but no studies have been conducted. We aimed first to explore overall extraradicular infection, as well as specifically by Porphyromonas spp; and their potential to translocate from infected root canals to blood through peripheral blood mononuclear cells. In this cross-sectional study, healthy individuals with and without a diagnosis of apical periodontitis with an associated apical lesion of endodontic origin (both, symptomatic and asymptomatic) were included. Apical lesions (N=64) were collected from volunteers with an indication of tooth extraction. Intracanal samples (N=39) and respective peripheral blood mononuclear cells from apical periodontitis (n=14) individuals with an indication of endodontic treatment, as well as from healthy individuals (n=14) were collected. The detection frequencies and loads (DNA copies/mg or DNA copies/mu L) of total bacteria, Porphyromonas endodontalis and Porphyromonas gingivalis were measured by qPCR. In apical lesions, the detection frequencies (%) and median bacterial loads (DNA copies/mg) respectively were 70.8% and 4521.6 for total bacteria; 21.5% and 1789.7 for Porphyromonas endodontalis; and 18.4% and 1493.9 for Porphyromonas gingivalis. In intracanal exudates, the detection frequencies and median bacterial loads respectively were 100% and 21089.2 (DNA copies/mu L) for total bacteria, 41% and 8263.9 for Porphyromonas endodontalis; and 20.5%, median 12538.9 for Porphyromonas gingivalis. Finally, bacteria were detected in all samples of peripheral blood mononuclear cells including apical periodontitis and healthy groups, though total bacterial loads (median DNA copies/mu L) were significantly higher in apical periodontitis (953.6) compared to controls (300.7), pPorphyromonas endodontalis was equally detected in both groups (50%), but its bacterial load tended to be higher in apical periodontitis (262.3) than controls (158.8), p>0.05; Porphyromonas gingivalis was not detected. Bacteria and specifically Porphyromonas spp. were frequently detected in endodontic canals and apical lesions. Also, total bacteria and Porphyromonas endodontalis DNA were detected in peripheral blood mononuclear cells, supporting their plausible role in bacterial systemic translocation.
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Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT 1160741
1200098
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Lenguage
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en
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Publisher
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Frontiers Media
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Type of license
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Attribution-NonCommercial-NoDerivs 3.0 United States