Postnatal cell proliferation in the hippocampus is modulated by dopaminergic systems in the rat : effect of perinatal asphyxia
Professor Advisor
dc.contributor.advisor
Morales Retamales, Paola
Professor Advisor
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Herrera-Marschitz Muller, Mario
Author
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Esmar Gutiérrez, Daniela Isabel
Admission date
dc.date.accessioned
2021-11-03T23:53:56Z
Available date
dc.date.available
2021-11-03T23:53:56Z
Publication date
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2013
Identifier
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https://repositorio.uchile.cl/handle/2250/182571
Abstract
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Postnatal neurogenesis has been observed in brain regions, such as dentate gyrus of the
hippocampus and subventricular zone of telencephalon. Postnatal neurogenesis is an active
phenomenon modulated by several factors including monoamine transmission. Mesencephalic
dopamine (DA) neurons have been observed to project to the hippocampus, although a
detailed map of that projection is still lacking, and the modulation of neurogenesis by DA is
still controversial. Neurocircuitries of the basal ganglia and hippocampus are vulnerable to
global anoxia/ischemia occurring at the neonatal stage.
We have investigated here on the DA modulation of postnatal cell proliferation and
neurogenesis in hippocampus using organotypic cultures from control and asphyxia-exposed
rats. Tissue from hippocampus, substantia nigra (SN) and/or ventral tegmental area (VTA)
was taken at P1-3 for preparing organotypic cultures, plated on a coverslip and cultured for
22-24 days. The cultures were treated with 5-bromo-2’deoxyuridine (BrdU, 10µM), a marker
of mitosis, and apomorphine (10mM), a DA agonist, for 48h. At P22-24, the cultures were
fixed and treated for immunocytochemistry, using selective antibodies for tyrosine
hydroxylase, microtubule-associated protein-2, and BrdU, evaluated with confocal
microscopy.
We found that: (i) DA neurons project to hippocampus, under in vivo and in vitro
conditions. (ii) Postnatal hippocampal cell proliferation occurs under in vivo and in vitro
conditions, both in control and asphyxia-exposed animals. In hippocampus monocultures cell
proliferation was increased in tissue from asphyxia-exposed animals when compared to the
control condition. However, cell proliferation was only increased in hippocampus/VTA
control cultures. (iii) Apomorphine treatment increased cell proliferation in hippocampal
monocultures from both control and asphyxia-exposed animals.
This study demonstrates the modulation of postnatal neurogenesis taking place in
hippocampus by DA, which is vulnerable to perinatal asphyxia.
es_ES
Lenguage
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es
es_ES
Publisher
dc.publisher
Universidad de Chile
es_ES
Type of license
dc.rights
Attribution-NonCommercial-NoDerivs 3.0 United States