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Authordc.contributor.authorGutiérrez Contreras, Esteban
Authordc.contributor.authorGlavic Maurer, Álvaro Alberto
Authordc.contributor.authorBrand, Andrea
Authordc.contributor.authorSierralta Jara, Jimena Alejandra
Admission datedc.date.accessioned2021-11-24T16:59:11Z
Available datedc.date.available2021-11-24T16:59:11Z
Publication datedc.date.issued2021
Cita de ítemdc.identifier.citationJournal of Neuroscience 28 July 2021, 41 (30) 6430-6448es_ES
Identifierdc.identifier.other10.1523/JNEUROSCI.0452-20.2021
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/182853
Abstractdc.description.abstractThe adaptable transcriptional response to changes in food availability not only ensures animal survival but also lets embryonic development progress. Interestingly, the CNS is preferentially protected from periods of malnutrition, a phenomenon known as "brain sparing." However, the mechanisms that mediate this response remain poorly understood. To get a better understanding of this, we used Drosophila melanogaster as a model, analyzing the transcriptional response of neural stem cells (neuroblasts) and glia of the blood-brain barrier (BBB) from larvae of both sexes during nutrient restriction using targeted DamID. We found differentially expressed genes in both neuroblasts and glia of the BBB, although the effect of nutrient deficiency was primarily observed in the BBB. We characterized the function of a nutritional sensitive gene expressed in the BBB, the serine protease homolog, scarface (scaf). Scaf is expressed in subperineurial glia in the BBB in response to nutrition. Tissue-specific knockdown of scaf increases subperineurial glia endoreplication and proliferation of perineurial glia in the blood-brain barrier. Furthermore, neuroblast proliferation is diminished on scaf knockdown in subperineurial glia. Interestingly, reexpression of Scaf in subperineurial glia is able to enhance neuroblast proliferation and brain growth of animals in starvation. Finally, we show that loss of scaf in the blood-brain barrier increases sensitivity to drugs in adulthood, suggesting a physiological impairment. We propose that Scaf integrates the nutrient status to modulate the balance between neurogenesis and growth of the BBB, preserving the proper equilibrium between the size of the barrier and the brain.es_ES
Patrocinadordc.description.sponsorshipRoyal Society Darwin Trust Research Professorship Wellcome Trust European Commission 103792 Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT 1171800 Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDAP 15090007 CONICYT PIA Grant ACT1401es_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherSoc. Neurosciencees_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
Sourcedc.sourceJournal of Neurosciencees_ES
Keywordsdc.subjectBlood-brain barrieres_ES
Keywordsdc.subjectDrosophila melanogasteres_ES
Keywordsdc.subjectGlial cellses_ES
Keywordsdc.subjectNutrient restrictiones_ES
Keywordsdc.subjectScarfacees_ES
Keywordsdc.subjectSerine protease homologes_ES
Títulodc.titleThe serine protease homolog, scarface, is sensitive to nutrient availability and modulates the development of the drosophila blood brain barrieres_ES
Document typedc.typeArtículo de revistaes_ES
dc.description.versiondc.description.versionVersión publicada - versión final del editores_ES
Date of embargodc.description.embargo28-01-2022es_ES
dcterms.accessRightsdcterms.accessRightsAcceso embargadoes_ES
Catalogueruchile.catalogadorcrbes_ES
Indexationuchile.indexArtículo de publícación WoSes_ES


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Attribution-NonCommercial-NoDerivs 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States