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Authordc.contributor.authorMaldonado Maldonado, Edio Luis
Authordc.contributor.authorMorales Pisón, Sebastián
Authordc.contributor.authorUrbina, Fabiola
Authordc.contributor.authorSolari Illescas, Aldo Gerónimo
Cita de ítemdc.identifier.citationFront. Cell. Infect. Microbiol., August 2021 Volume 11 Article 670564es_ES
Abstractdc.description.abstractTrypanosomatids are a group of primitive unicellular eukaryotes that can cause diseases in plants, insects, animals, and humans. Kinetoplast genome integrity is key to trypanosomatid cell survival and viability. Kinetoplast DNA (kDNA) is usually under attack by reactive oxygen and nitric species (ROS and RNS), damaging the DNA, and the cells must remove and repair those oxidatively generated lesions in order to survive and proliferate. Base excision repair (BER) is a well-conserved pathway for DNA repair after base damage, single-base loss, and single-strand breaks, which can arise from ROS, RSN, environmental genotoxic agents, and UV irradiation. A powerful BER system has been described in the T. cruzi kinetoplast and it is mainly carried out by DNA polymerase beta (pol beta) and DNA polymerase beta-PAK (pol beta-PAK), which are kinetoplast-located in T. cruzi as well as in other trypanosomatids. Both pol b and pol beta-PAK belong to the X-family of DNA polymerases (pol X family), perform BER in trypanosomatids, and display intrinsic 5-deoxyribose phosphate (dRP) lyase and DNA polymerase activities. However, only Pol beta-PAK is able to carry out trans-lesion synthesis (TLS) across 8oxoG lesions. T. cruzi cells overexpressing pol beta are more resistant to ROS and are also more efficient to repair 8oxoG compared to control cells. Pol beta seems to play a role in kDNA replication, since it associates with kinetoplast antipodal sites in those development stages in trypanosomatids which are competent for cell replication. ROS treatment of cells induces the overexpression of pol beta, indicating that plays a role in kDNA repair. In this review, we will summarize the main features of trypanosomatid minicircle kDNA replication and the biochemical characteristics of pol beta-like enzymes and their involvement in BER and kDNA replication. We also summarize key structural features of trypanosomatid pol beta compared to their mammalian (human) counterpart.es_ES
Patrocinadordc.description.sponsorshipComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT 1190392 ICBM, Faculty of Medicine, the University of Chilees_ES
Publisherdc.publisherFrontiers Mediaes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
Link to Licensedc.rights.uri*
Sourcedc.sourceFrontiers in Cellular and Infection Microbiologyes_ES
Keywordsdc.subjectTrypanosoma cruzies_ES
Keywordsdc.subjectDNA polymerase betaes_ES
Keywordsdc.subjectKinetoplast DNAes_ES
Títulodc.titleMolecular and functional characteristics of DNA polymerase beta-like enzymes from trypanosomatidses_ES
Document typedc.typeArtículo de revistaes_ES
dc.description.versiondc.description.versionVersión publicada - versión final del editores_ES
dcterms.accessRightsdcterms.accessRightsAcceso abiertoes_ES
Indexationuchile.indexArtículo de publícación WoSes_ES

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States