Trypanosoma cruzi infection follow-up in a sylvatic vector of Chagas disease: comparing early and late stage nymphs
Author
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Cortés, Valeria
Author
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Cruz, Amalia
Author
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Onetti, Sofía
Author
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Kinzel, Daniela
Author
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García, Javiera
Author
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Ortiz, Sylvia
Author
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López, Angélica
Author
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Cattan Ayala, Pedro Eduardo
Author
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Botto Mahan, Carezza Verónica
Author
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Solari Illescas, Aldo Gerónimo
Admission date
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2021-12-16T20:22:02Z
Available date
dc.date.available
2021-12-16T20:22:02Z
Publication date
dc.date.issued
2021
Cita de ítem
dc.identifier.citation
PLoS Negl Trop Dis 15(9): e0009729 2021
es_ES
Identifier
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10.1371/journal.pntd.0009729
Identifier
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https://repositorio.uchile.cl/handle/2250/183277
Abstract
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Chagas disease is caused by Trypanosoma cruzi and transmitted by the triatomine Mepraia
spinolai in the southwest of South America. Here, we examined the T. cruzi-infection
dynamics of field-caught M. spinolai after laboratory feeding, with a follow-up procedure on
bug populations collected in winter and spring of 2017 and 2018. Bugs were analyzed twice
to evaluate T. cruzi-infection by PCR assays of urine/fecal samples, the first evaluation right
after collection and the second 40 days after the first feeding. We detected bugs with: the
first sample positive and second negative (+/-), the first sample negative and second positive (-/+), and with both samples positive or negative (+/+; -/-). Bugs that resulted positive on
both occasions were the most frequent, with the exception of those collected in winter 2018.
Infection rate in spring was higher than winter only in 2018. Early and late stage nymphs presented similar T. cruzi-infection rates except for winter 2017; therefore, all nymphs may contribute to T. cruzi-transmission to humans. Assessment of infection using two samples
represents a realistic way to determine the infection a triatomine can harbor. The underlying
mechanism may be that some bugs do not excrete parasites unless they are fed and maintained for some time under environmentally controlled conditions before releasing T. cruzi,
which persists in the vector hindgut. We suggest that T. cruzi-infection dynamics regarding
the three types of positive-PCR results detected by follow-up represent: residual T. cruzi in
the rectal lumen (+/-), colonization of parasites attached to the rectal wall (-/+), and presence
of both kinds of flagellates in the hindgut of triatomines (+/+). We suggest residual T. cruziinfections are released after feeding, and result 60–90 days after infection persisting in the
rectal lumen after a fasting event, a phenomenon that might vary between contrasting seasons and years.
es_ES
Patrocinador
dc.description.sponsorship
National Research and Development Agency- National Fund for Scientific and Technological Development 1190392
National Research and Development AgencyNational Fund for Scientific and Technological Development 1170367
National Research and Development Agency-National Fund for Scientific and Technological Development 1180940
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Lenguage
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en
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Publisher
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Public Library Science
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Type of license
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Attribution-NonCommercial-NoDerivs 3.0 United States