In vitro effect of copper (I) complex [Cu (NN1)2] (ClO4) on vibrio harveyi BB170 biofilm formation
Author
dc.contributor.author
Soto Aguilera, Sarita Elizabeth
Author
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Modak, Brenda
Author
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Aldabaldetrecu, Maialen
Author
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Lozano Moraga, Carla Paola
Author
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Guerrero, Juan
Author
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Lefimil Puente, Claudia Andrea
Author
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Parra, Mick
Admission date
dc.date.accessioned
2022-06-01T15:05:40Z
Available date
dc.date.available
2022-06-01T15:05:40Z
Publication date
dc.date.issued
2021
Cita de ítem
dc.identifier.citation
Microorganisms 2021, 9, 2273.
es_ES
Identifier
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10.3390/microorganisms9112273
Identifier
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https://repositorio.uchile.cl/handle/2250/185807
Abstract
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Biofilm formation in pathogenic bacteria is an important factor of resistance to antimicrobial
treatments, allowing them to survive for a long time in their hosts. In the search for new antibiofilm
agents, in this work we report the activity of a copper (I) complex, [Cu(NN1)2]ClO4, synthesized with
Cu (I) and NN1, an imine ligand 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one, a derivate
of natural compound coumarin. The antibacterial and antibiofilm capacity was evaluated in Vibrio
harveyi BB170 used as model bacteria. Antibacterial activity was measured in vitro by minimal inhibitory
concentration (MIC), minimal bactericidal concentration (MBC) and half-maximal inhibitory
concentration (IC50) determination. Antibiofilm capacity of copper (I) complex was analyzed by different
concentrations of IC50 values. The results showed that the sub-IC50 concentration, 12.6 g/mL
of the copper (I) complex, was able to reduce biofilm formation by more than 75%, and bacterial
viability was reduced by 50%. Inverted and confocal laser scanning microscopy showed that the
[Cu(NN1)2]ClO4 complex affected the biofilm structure. Therefore, the copper (I) complex is effective
as an antibiofilm compound in V. harveyi BB170.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT 1180265
1191902
11150928
FONDEQUIP EQM 150106
150069
USACH
National Doctorate Scholarship 21212170
EQM 150069
es_ES
Lenguage
dc.language.iso
en
es_ES
Publisher
dc.publisher
MDPI
es_ES
Type of license
dc.rights
Attribution-NonCommercial-NoDerivs 3.0 United States