Human papillomavirus detected in oropharyngeal cancers from chilean subjects
Author
dc.contributor.author
Oliva Guerrero, Carolina
Author
dc.contributor.author
Carrillo Beltrán, Diego
Author
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Boettiger Bonnefoy, Paul
Author
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Gallegos Méndez, Iván
Author
dc.contributor.author
Aguayo González, Francisco
Admission date
dc.date.accessioned
2022-07-18T19:23:19Z
Available date
dc.date.available
2022-07-18T19:23:19Z
Publication date
dc.date.issued
2022
Cita de ítem
dc.identifier.citation
Viruses 2022, 14, 1212.
es_ES
Identifier
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10.3390/v14061212
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/186808
Abstract
dc.description.abstract
High-risk human papillomaviruses (HR-HPV) are the causal agents of an important subset of oropharyngeal cancers that has increased considerably in incidence in recent years. In this study, we evaluated the presence of HPV in 49 oropharyngeal cancers from Chilean subjects. The presence of HPV DNA was analyzed by conventional PCR, the genotypes were identified through sequencing, and the expression of E6/E7 transcripts was evaluated by a reverse transcriptase polymerase chain reaction (RT-PCR). Additionally, to determine p16 expression-a surrogate marker for oncogenic HPV infection-a tissue array was constructed for immunohistochemistry (IHC). HPV was detected in 61.2% of oropharyngeal carcinomas, the most prevalent genotype being HPV16 (80%). E6 and E7 transcripts were detected in 91.6% and 79.1% of the HPV16-positive specimens, respectively, demonstrating functional HPV infections. Furthermore, p16 expression was positive in 58.3% of cases. These findings show a high prevalence of HR-HPV in oropharyngeal tumors from Chile, suggesting the necessity of additional studies to address this growing public health concern.
es_ES
Patrocinador
dc.description.sponsorship
Chilean Society of Otorhinolaryngology 21181116
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT 1161219
1221033
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDAP
15130011
es_ES
Lenguage
dc.language.iso
en
es_ES
Publisher
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MDPI
es_ES
Type of license
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Attribution-NonCommercial-NoDerivs 3.0 United States