A dual treatment blocks alcohol binge-drinking relapse: microbiota as a new player
Author
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Ezquer, Fernando
Author
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Quintanilla González, María Elena
Author
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Morales Retamales, Eugenia
Author
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Santapaul, Daniela
Author
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Munita, José Manuel
Author
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Moya Flores, Francisco
Author
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Ezquer, Marcelo
Author
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Herrera Marschitz, Mario
Author
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Israel Jacard, Yedy
Admission date
dc.date.accessioned
2022-07-18T20:09:05Z
Available date
dc.date.available
2022-07-18T20:09:05Z
Publication date
dc.date.issued
2022
Cita de ítem
dc.identifier.citation
Drug and Alcohol Dependence 236 (2022) 109466
es_ES
Identifier
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10.1016/j.drugalcdep.2022.109466
Identifier
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https://repositorio.uchile.cl/handle/2250/186811
Abstract
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Rationale: Gut microbiota communicates information to the brain. Some animals are born with a gut microbiota that predisposes to high alcohol consumption, and transplantation of fecal material from alcoholics to mice increases animal preference for ethanol. Alcohol-use-disorders are chronic conditions where relapse is the hallmark. A predictive animal model of relapse is the "alcohol deprivation effect " where ethanol re-access is allowed following chronic alcohol intake and a long alcohol deprivation. The present study evaluates the effect of gut microbiota modification on relapse, as an adjunct to N-acetylcysteine + Acetylsalicylic acid administration, which inhibits the alcohol-induced hyper-glutamatergic condition.
Methods: Rats bred as heavy alcohol consumers (UChB) were allowed ethanol intake for one month, were deprived of alcohol for two-weeks and subsequently offered re-access to ethanol. Prior to ethanol re-access animals received orally either (i) vehicle-control, (ii) Lactobacillus-rhamnosus-GG after antibiotic treatment (LGG); (iii) N-acetylcysteine+Acetylsalicylic acid (NAC/ASA) or (iv) both treatments: LGG+ (NAC/ASA).
Results: Marked binge drinking (1.75 g ethanol/kg in 60 min) and blood alcohol levels exceeding 80 mg/dl were observed in the control group upon ethanol-re-access. Lactobacillus-GG or (NAC+ASA) treatments inhibited alcohol intake by 66-80%. The combination of both treatments virtually suppressed (inhibition of 90%) the re access binge-like drinking, showing additive effects. Treatment with NAC+ASA increased the levels of glutamate transporters xCT and GLT-1 in nucleus accumbens, while Lactobacillus-GG administration increased those of the dopamine transporter (DAT).
Conclusions: The administration of a well-accepted probiotic may be of value as an adjunct in the treatment of alcohol-use-disorders.
es_ES
Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT 1200287
ANID-ACT210012
es_ES
Lenguage
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en
es_ES
Publisher
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Elsevier
es_ES
Type of license
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Attribution-NonCommercial-NoDerivs 3.0 United States