The catalytic subunit of schizosaccharomyces pombe CK2 (Cka1) negatively regulates RNA polymerase II transcription through phosphorylation of positive cofactor 4 (PC4)
Author
dc.contributor.author
Rojas, Diego A.
Author
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Urbina, Fabiola
Author
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Solari Illescas, Aldo Gerónimo
Author
dc.contributor.author
Maldonado Maldonado, Edio Luis
Admission date
dc.date.accessioned
2023-06-14T21:16:32Z
Available date
dc.date.available
2023-06-14T21:16:32Z
Publication date
dc.date.issued
2022
Cita de ítem
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Int. J. Mol. Sci. 2022, 23, 9499.
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Identifier
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10.3390/ijms23169499
Identifier
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https://repositorio.uchile.cl/handle/2250/194337
Abstract
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Positive cofactor 4 (PC4) is a transcriptional coactivator that plays important roles in
transcription and DNA replication. In mammals, PC4 is phosphorylated by CK2, and this event
downregulates its RNA polymerase II (RNAPII) coactivator function. This work describes the effect of
fission yeast PC4 phosphorylation on RNAPII transcription in a cell extract, which closely resembles
the cellular context. We found that fission yeast PC4 is strongly phosphorylated by the catalytic
subunit of CK2 (Cka1), while the regulatory subunit (Ckb1) downregulates the PC4 phosphorylation.
The addition of Cka1 to an in vitro transcription assay can diminish the basal transcription from the
Ad-MLP promoter; however, the addition of recombinant fission yeast PC4 or Ckb1 can stimulate
the basal transcription in a cell extract. Fission yeast PC4 is phosphorylated in a domain which
has consensus phosphorylation sites for CK2, and two serine residues were identified as critical for
CK2 phosphorylation. Mutation of one of the serine residues in PC4 does not completely abolish
the phosphorylation; however, when the two serine residues are mutated, CK2 is no longer able
to phosphorylate PC4. The mutant which is not phosphorylated is able to stimulate transcription
even though it is previously phosphorylated by Cka1, while the wild type and the point mutant are
inactivated by Cka1 phosphorylation, and they cannot stimulate transcription by RNAPII in cell
extracts. Those results demonstrate that CK2 can regulate the coactivator function of fission yeast
PC4 and suggests that this event could be important in vivo as well.
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Patrocinador
dc.description.sponsorship
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT 11191121
1190392
Instituto de Ciencias Biomedicas (ICBM)
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Lenguage
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en
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Publisher
dc.publisher
MDPI
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Type of license
dc.rights
Attribution-NonCommercial-NoDerivs 3.0 United States
The catalytic subunit of schizosaccharomyces pombe CK2 (Cka1) negatively regulates RNA polymerase II transcription through phosphorylation of positive cofactor 4 (PC4)