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Authordc.contributor.authorAbrigo, Johanna
Authordc.contributor.authorOlguín, Hugo
Authordc.contributor.authorGutiérrez, Danae
Authordc.contributor.authorTacchi, Franco
Authordc.contributor.authorArrese, Marco
Authordc.contributor.authorCabrera, Daniel
Authordc.contributor.authorValero Breton, Mayalan
Authordc.contributor.authorElorza, Alvaro A.
Authordc.contributor.authorSimon Pino, Felipe Alonso
Authordc.contributor.authorCabello Verrugio, Claudio
Admission datedc.date.accessioned2023-07-18T17:17:22Z
Available datedc.date.available2023-07-18T17:17:22Z
Publication datedc.date.issued2022
Cita de ítemdc.identifier.citationAntioxidants 2022, 11, 1706es_ES
Identifierdc.identifier.other10.3390/antiox11091706
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/194796
Abstractdc.description.abstractCholestatic chronic liver disease is characterized by developing sarcopenia and elevated serum levels of bile acids. Sarcopenia is a skeletal muscle disorder with the hallmarks of muscle weakness, muscle mass loss, and muscle strength decline. Our previous report demonstrated that deoxycholic acid (DCA) and cholic acid (CA), through the membrane receptor TGR5, induce a sarcopenia-like phenotype in myotubes and muscle fibers. The present study aimed to evaluate the impact of DCA and CA on mitochondrial mass and function in muscle fibers and the role of the TGR5 receptor. To this end, muscle fibers obtained from wild-type and TGR5(-/-) mice were incubated with DCA and CA. Our results indicated that DCA and CA decreased mitochondrial mass, DNA, and potential in a TGR5-dependent fashion. Furthermore, with TGR5 participation, DCA and CA also reduced the oxygen consumption rate and complexes I and II from the mitochondrial electron transport chain. In addition, DCA and CA generated more mitochondrial reactive oxygen species than the control, which were abolished in TGR5(-/-) mice muscle fibers. Our results indicate that DCA and CA induce mitochondrial dysfunction in muscle fibers through a TGR5-dependent mechanism.es_ES
Patrocinadordc.description.sponsorshipNational Fund for Science and Technological Development (FONDECYT) 1200944 1201039 1191145 1211879 1180983 Agencia Nacional de Investigacion y Desarrollo (ANID)-Millennium Science Initiative Program ICN09_016/ICN 2021_045 BASAL Grant-CEDENNA from the National Research and Development Agency (ANID), Government of Chile AFB180001 BASAL Grant from the National Research and Development Agency (ANID), Government of Chile, CARE Chile UC AFB170005 National Research and Development Agency ANID ACE 210009 European Union's Horizon 2020 Research and Innovation Program 825510 Iniciativa Cientifica Milenio (ANID, Chile) ANID VRID-UNAB Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT 1200944es_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherMDPIes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
Sourcedc.sourceAntioxidantses_ES
Keywordsdc.subjectBile acidses_ES
Keywordsdc.subjectMitochondriaes_ES
Keywordsdc.subjectSarcopeniaes_ES
Keywordsdc.subjectMuscle wastinges_ES
Keywordsdc.subjectTGR5 receptores_ES
Títulodc.titleBile acids induce alterations in mitochondrial function in skeletal muscle fiberses_ES
Document typedc.typeArtículo de revistaes_ES
dc.description.versiondc.description.versionVersión publicada - versión final del editores_ES
dcterms.accessRightsdcterms.accessRightsAcceso abiertoes_ES
Catalogueruchile.catalogadorapces_ES
Indexationuchile.indexArtículo de publícación WoSes_ES


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Attribution-NonCommercial-NoDerivs 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States