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Authordc.contributor.authorBlanco Santana, Rancés
Authordc.contributor.authorCarrillo Beltrán, Diego Antonio
Authordc.contributor.authorMuñoz, Juan P.
Authordc.contributor.authorOsorio, Julio C.
Authordc.contributor.authorTapia Pineda, Julio César
Authordc.contributor.authorBurzi, Verónica A.
Authordc.contributor.authorGallegos Méndez, Iván Marcelo
Authordc.contributor.authorCalaf, Gloria M.
Authordc.contributor.authorChabay, Paola
Authordc.contributor.authorAguayo González, Francisco Renán
Cita de ítemdc.identifier.citationMicroorganisms 2022, 10, 888es_ES
Abstractdc.description.abstractHigh-risk human papillomaviruses (HR-HPVs) are the etiological agents of cervical cancer. However, a low proportion of HR-HPV-infected women finally develop this cancer, which suggests the involvement of additional cofactors. Epstein-Barr virus (EBV) has been detected in cervical squamous cell carcinomas (SCCs) as well as in low- (LSIL) and high-grade (HSIL) squamous intraepithelial lesions, although its role is unknown. In this study, we characterized HR-HPV/EBV co-presence and viral gene expression in LSIL (n = 22), HSIL (n = 52), and SCC (n = 19) from Chilean women. Additionally, phenotypic changes were evaluated in cervical cancer cells ectopically expressing BamHI-A Rightward Frame 1 (BARF1). BARF1 is a lytic gene also expressed in EBV-positive epithelial tumors during the EBV latency program. HPV was detected in 6/22 (27.3%) LSIL, 38/52 (73.1%) HSIL, and 15/19 (78.9%) SCC cases (p < 0.001). On the other hand, EBV was detected in 16/22 (72.7%) LSIL, 27/52 (51.9%) HSIL, and 13/19 (68.4%) SCC cases (p = 0.177). HR-HPV/EBV co-presence was detected in 3/22 (13.6%) LSIL, 17/52 (32.7%) HSIL, and 11/19 (57.9%) SCC cases (p = 0.020). Additionally, BARF1 transcripts were detected in 37/55 (67.3%) of EBV positive cases and in 19/30 (63.3%) of HR-HPV/EBV positive cases. Increased proliferation, migration, and epithelial-mesenchymal transition (EMT) was observed in cervical cancer cells expressing BARF1. Thus, both EBV and BARF1 transcripts are detected in low- and high-grade cervical lesions as well as in cervical carcinomas. In addition, BARF1 can modulate the tumor behavior in cervical cancer cells, suggesting a role in increasing tumor aggressiveness.es_ES
Patrocinadordc.description.sponsorshipANID/FONDECYT Postdoctoral 3190723 Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT 1221033 1220353 Lineas de apoyo a la investigacion financiadas por el ICBM Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDAP 15130011es_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
Link to Licensedc.rights.uri*
Keywordsdc.subjectEpstein-barr viruses_ES
Keywordsdc.subjectHuman papillomaviruses_ES
Keywordsdc.subjectBamHI-A rightward frame 1 expressiones_ES
Keywordsdc.subjectCervical canceres_ES
Títulodc.titleCharacterization of high-risk HPV/EBV co-presence in pre-malignant cervical lesions and squamous cell carcinomases_ES
Document typedc.typeArtículo de revistaes_ES
dc.description.versiondc.description.versionVersión publicada - versión final del editores_ES
dcterms.accessRightsdcterms.accessRightsAcceso abiertoes_ES
Indexationuchile.indexArtículo de publícación WoSes_ES

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