Transplantation-Associated Long-Term Immunosuppression Promotes Oral Colonization by Potentially Opportunistic Pathogens without Impacting Other Members of the Salivary Bacteriome
Author
dc.contributor.author
Díaz, Patricia I.
Author
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Hong, Bo Young
es_CL
Author
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Frias Lopez, Jorge
es_CL
Author
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Dupuy, Amanda K.
es_CL
Author
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Angeloni, Mark
es_CL
Author
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Abusleme Ramos, Loreto Andrea
es_CL
Author
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Terzi, Ioannidou
es_CL
Author
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Ioannidou, Effie
es_CL
Author
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Strausbaugh, Linda D.
es_CL
Author
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Dongari Bagtzoglou, Anna
Admission date
dc.date.accessioned
2014-01-30T19:32:12Z
Available date
dc.date.available
2014-01-30T19:32:12Z
Publication date
dc.date.issued
2013
Cita de ítem
dc.identifier.citation
Clin. Vaccine Immunol. 2013, 20(6):920
en_US
Identifier
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doi: 10.1128/CVI.00734-12
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/123521
General note
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Artículo de publicación ISI
en_US
Abstract
dc.description.abstract
Solid-organ transplant recipients rely on pharmacological immunosuppression to prevent allograft rejection. The effect of such
chronic immunosuppression on the microflora at mucosal surfaces is not known. We evaluated the salivary bacterial microbiome
of 20 transplant recipients and 19 nonimmunosuppressed controls via 454 pyrosequencing of 16S rRNA gene amplicons.
Alpha-diversity and global community structure did not differ between transplant and control subjects. However, principal coordinate
analysis showed differences in community membership. Taxa more prevalent in transplant subjects included operational
taxonomic units (OTUs) of potentially opportunistic Gammaproteobacteria such as Klebsiella pneumoniae, Pseudomonas
fluorescens, Acinetobacter species, Vibrio species, Enterobacteriaceae species, and the genera Acinetobacter and Klebsiella. Transplant
subjects also had increased proportions of Pseudomonas aeruginosa, Acinetobacter species, Enterobacteriaceae species, and
Enterococcus faecalis, among other OTUs, while genera with increased proportions included Klebsiella, Acinetobacter, Staphylococcus,
and Enterococcus. Furthermore, in transplant subjects, the dose of the immunosuppressant prednisone positively correlated
with bacterial richness, while prednisone and mycophenolate mofetil doses positively correlated with the prevalence and
proportions of transplant-associated taxa. Correlation network analysis of OTU relative abundance revealed a cluster containing
potentially opportunistic pathogens as transplant associated. This cluster positively correlated with serum levels of C-reactive
protein, suggesting a link between the resident flora at mucosal compartments and systemic inflammation. Network connectivity
analysis revealed opportunistic pathogens as highly connected to each other and to common oral commensals, pointing to
bacterial interactions that may influence colonization. This work demonstrates that immunosuppression aimed at limiting Tcell-
mediated responses creates a more permissive oral environment for potentially opportunistic pathogens without affecting
other members of the salivary bacteriome.
Transplantation-Associated Long-Term Immunosuppression Promotes Oral Colonization by Potentially Opportunistic Pathogens without Impacting Other Members of the Salivary Bacteriome