| Author | dc.contributor.author | Diez, Cristina | |
| Author | dc.contributor.author | Lorenz, Virginia | es_CL |
| Author | dc.contributor.author | Ortiz, Silvia | es_CL |
| Author | dc.contributor.author | González, Verónica | es_CL |
| Author | dc.contributor.author | Racca, Andrea | es_CL |
| Author | dc.contributor.author | Bontempi, Iván | es_CL |
| Author | dc.contributor.author | Manattini, Silvia | es_CL |
| Author | dc.contributor.author | Solari Illescas, Aldo | es_CL |
| Author | dc.contributor.author | Marcipar, Iván | es_CL |
| Admission date | dc.date.accessioned | 2010-07-14T13:25:07Z | |
| Available date | dc.date.available | 2010-07-14T13:25:07Z | |
| Publication date | dc.date.issued | 2010 | |
| Cita de ítem | dc.identifier.citation | Am. J. Trop. Med. Hyg., 82(1), 2010, pp. 67–73 | en_US |
| Identifier | dc.identifier.other | doi:10.4269/ajtmh.2010.09-0391 | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/128690 | |
| Abstract | dc.description.abstract | We have evaluated blood samples of chronic and congenital Trypanosoma cruzi -infected patients from the
city of Reconquista located in the northeast of Argentina where no information was previously obtained about the genotype
of infecting parasites. Fourteen samples of congenital and 19 chronical patients were analyzed by hybridization with
DNA probes of minicircle hypervariable regions (mHVR). In congenital patients, 50% had single infections with TcIId,
7% single infections with TcIIe, 29% mixed infections with TcIId/e, and 7% had mixed infections with TcIId/b and 7%
TcIId/b, respectively. In Chronical patients, 52% had single infections with TcIId, 11% single infections with TcIIe, 26%
had mixed infections with TcIId/e, and 11% had non-identified genotypes. With these samples, we evaluated the minicircle
lineage-specific polymerase chain reaction assay (MLS-PCR), which involves a nested PCR to HVR minicircle sequences
and we found a correlation with hybridization probes of 96.4% for TcIId and 54.8% for TcIIe. | en_US |
| Patrocinador | dc.description.sponsorship | This work received financial support from CAI+D
2009 PI 15-86, Universidad Nacional del Litoral and FONDECYTChile
1085154. | en_US |
| Lenguage | dc.language.iso | en | en_US |
| Título | dc.title | Genotyping of Trypanosoma cruzi Sublineage in Human Samples from a North-East Argentina Area by Hybridization with DNA Probes and Specific Polymerase Chain Reaction (PCR) | en_US |
| Document type | dc.type | Artículo de revista | |
