Oxidative response of neutrophils to platelet-activating factor is altered during acute ruminal acidosis induced by oligofructose in heifers
Author
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Concha, Claudia
Author
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Carretta, María Daniella
es_CL
Author
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Alarcón, Pablo
es_CL
Author
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Conejeros, Iván
es_CL
Author
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Gallardo, Diego
es_CL
Author
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Hidalgo, Alejandra Isabel
es_CL
Author
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Tadich, Nestor
es_CL
Author
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Cáceres Lillo, Dante
es_CL
Author
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Hidalgo, María Angélica
es_CL
Author
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Burgos, Rafael Agustín
es_CL
Admission date
dc.date.accessioned
2014-12-16T18:54:36Z
Available date
dc.date.available
2014-12-16T18:54:36Z
Publication date
dc.date.issued
2014
Cita de ítem
dc.identifier.citation
J. Vet. Sci. (2014), 15(2), 217-224
en_US
Identifier
dc.identifier.issn
1229-845X
Identifier
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dx.doi.org/10.4142/jvs.2014.15.2.217
Identifier
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https://repositorio.uchile.cl/handle/2250/129402
General note
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Artículo de publicación ISI
en_US
Abstract
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Reactive oxygen species (ROS) production is one of the main
mechanisms used to kill microbes during innate immune
response. D-lactic acid, which is augmented during acute
ruminal acidosis, reduces platelet activating factor
(PAF)-induced ROS production and L-selectin shedding in
bovine neutrophils in vitro. This study was conducted to
investigate whether acute ruminal acidosis induced by acute
oligofructose overload in heifers interferes with ROS
production and L-selectin shedding in blood neutrophils.
Blood neutrophils and plasma were obtained by jugular
venipuncture, while ruminal samples were collected using
rumenocentesis. Lactic acid from plasma and ruminal samples
was measured by HPLC. PAF-induced ROS production and
L-selectin shedding were measured in vitro in bovine
neutrophils by a luminol chemiluminescence assay and flow
cytometry, respectively. A significant increase in ruminal and
plasma lactic acid was recorded in these animals. Specifically,
a decrease in PAF-induced ROS production was observed 8 h
after oligofructose overload, and this was sustained until 48 h
post oligofructose overload. A reduction in PAF-induced
L-selectin shedding was observed at 16 h and 32 h post
oligofructose overload. Overall, the results indicated that
neutrophil PAF responses were altered in heifers with ruminal
acidosis, suggesting a potential dysfunction of the innate
immune response.
en_US
Patrocinador
dc.description.sponsorship
This work was supported by Fondo de Fomento al
Desarrollo Científico y Tecnológico (FONDECYT) grants
1090401 and 1120718.