Author | dc.contributor.author | Favaro Trombone, Ana Paula | |
Author | dc.contributor.author | Cavalla Ruiz, Ian Franco | |
Author | dc.contributor.author | Varize Silveira, Elcia María | |
Author | dc.contributor.author | Andreo, Camile Bermejo | |
Author | dc.contributor.author | Francisconi, Carolina Favaro | |
Author | dc.contributor.author | Fonseca, Angélica Cristina | |
Author | dc.contributor.author | Letra, Ariadne | |
Author | dc.contributor.author | Silva, Renato Menezes | |
Author | dc.contributor.author | Garlet, Gustavo Pompermaier | |
Admission date | dc.date.accessioned | 2017-03-01T19:11:05Z | |
Available date | dc.date.available | 2017-03-01T19:11:05Z | |
Publication date | dc.date.issued | 2016 | |
Cita de ítem | dc.identifier.citation | Journal of Applied Oral Science. Volumen: 24 Número: 4 Páginas: 366-375 | es_ES |
Identifier | dc.identifier.other | 10.1590/1678.775720160112 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/142834 | |
Abstract | dc.description.abstract | Increased matrix metalloproteinases (MMPs) activity is a hallmark of periapical granulomas. However, the factors underlying the MMPs expression modulation in healthy and diseased periapical tissues remains to be determined. Objective: In this study, we evaluated the association between the MMP1-1607 polymorphism (rs1799750) and pro-inflammatory milieu elements with MMP-1 mRNA levels in vivo. Material and Methods: MMP1-1607 SNP and the mRNA levels of MMP-1, TNF-alpha, IFN-gamma, IL-17A, IL-21, IL-10, IL-4, IL-9, and FOXp3 were determined via RealTimePCR in DNA/RNA samples from patients presenting periapical granulomas (N=111, for both genotyping and expression analysis) and control subjects (N=214 for genotyping and N=26 for expression analysis). The Shapiro-Wilk, Fisher, Pearson, Chi-square ordinal least squares regression tests were used for data analysis (p<0.05 was considered statistically significant). Results: The MMP1-1607 1G/2G and 1G/2G+2G/2G genotypes were significantly more prevalent in the patients than in controls, comprising a risk factor for periapical lesions development. MMP-1 mRNA levels were higher in periapical lesions than in healthy periodontal ligament samples, as well as higher in active than in inactive lesions. The polymorphic allele 2G carriers presented a significantly higher MMP-1 mRNA expression when compared with the 1G/1G genotype group. The ordered logistic regression demonstrated a significant correlation between the genetic polymorphism and the expression levels of MMP-1. Additionally, the pro- and anti-inflammatory cytokines IL-17A, IFN-gamma, TNF-alpha, IL-21, IL-10, IL-9, and IL-4 were significant as complementary explanatory variables of MMP-1 expression. Conclusion: The MMP1-1607 SNP was identified as a risk factor for periapical lesions development, possibly due to its association with increased MMP-1 mRNA levels in periapical lesions. The MMP-1 expression is also under the control of the inflammatory milieu elements, being the cytokines TNF-alpha, IL-21, IL-17A, and IFN-g associated with increased MMP-1 levels in periapical lesions, while IL-10, IL-9, or IL-4 presented an inverse association. | es_ES |
Lenguage | dc.language.iso | en | es_ES |
Type of license | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | * |
Link to License | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | * |
Source | dc.source | Journal of Applied Oral Science | es_ES |
Keywords | dc.subject | Cytokines | es_ES |
Keywords | dc.subject | Inflammation | es_ES |
Keywords | dc.subject | Genetic | es_ES |
Keywords | dc.subject | Polymorphism | es_ES |
Keywords | dc.subject | Matrix metalloproteinases | es_ES |
Keywords | dc.subject | Periapical granuloma | es_ES |
Keywords | dc.subject | Periapical diseases | es_ES |
Título | dc.title | MMP1-1607 polymorphism increases the risk for periapical lesion development through the upregulation MMP-1 expression in association with pro-inflammatory milieu elements | es_ES |
Document type | dc.type | Artículo de revista | |
Cataloguer | uchile.catalogador | C. R. B. | es_ES |
Indexation | uchile.index | Artículo de publicación ISI | es_ES |