In vitro segmentation induction of Mesocestoides corti (cestoda) tetrathyridia
Author
dc.contributor.author
Markoski, Melissa M.
Author
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Bizarro, Cristiano V.
Author
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Farias, Sandra
Author
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Espinoza, Ingrid
Author
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Galanti Garrone, Norbel
Author
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Zaha, Arnaldo
Author
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Ferreira, Henrique B.
Admission date
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2019-01-29T17:51:58Z
Available date
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2019-01-29T17:51:58Z
Publication date
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2003
Cita de ítem
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Journal of Parasitology, Volumen 89, Issue 1, 2018, Pages 27-34
Identifier
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00223395
Identifier
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10.1645/0022-3395(2003)089[0027:IVSIOM]2.0.CO;2
Identifier
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https://repositorio.uchile.cl/handle/2250/163623
Abstract
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Mesocestoides corti is a suitable model for studying cestode development because of its ability to reproduce asexually and segment in vitro. The cultured parasite is also capable of sexual differentiation and, probably, reproduction. To establish conditions that increase the efficiency of in vitro M. corti larvae (tetrathyridia) segmentation, we tested the effects of an inducing agent and some physical parameters in cultures. We found that a 5% CO2-95% N2 gas phase, an incubation temperature of 39 C (instead of 37 C), and a 24-hr pretreatment with trypsin (105 BAEE/ml, BAEE = Nα-benzoil-L-arginine ethyl ester unit of trypsin activity) in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 20% fetal bovine serum (FBS) are able to increase individually or synergistically the segmentation rate of tetrathyridia. A segmentation rate of up to 100% was achieved on day 4 of culture, when all these conditions were used simultaneously, in comparison with an average rate of 40% o