Pathogenicity island region of clinical and environmental strains of Vibrio parahaemolyticus, isolated in Chile Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
Author
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Núñez, Harold
Author
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Ulloa, María Teresa
Author
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Guerra, Fabiola
Author
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Osorio, Carlos G.
Admission date
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2019-03-11T12:57:38Z
Available date
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2019-03-11T12:57:38Z
Publication date
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2009
Cita de ítem
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Revista Medica de Chile, Volumen 137, Issue 2, 2018, Pages 208-214
Identifier
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00349887
Identifier
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07176163
Identifier
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https://repositorio.uchile.cl/handle/2250/164788
Abstract
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Background: Most clinical isolates of Vibrio parahaemolyticus produce a major virulence factor known as the thermostable direct hemolysin (TDH). TDH is encoded by the tdh gene which is located in a genomic pathogenicity island (PAI). Most environmental isolates are described as tdh negative. Aim: To assess if environmental strains lack the full pathogenicity island or if only the tdh gene is deleted. Material and methods: Thirty eight clinical and 66 environmental strains of Vibrio parahaemolyticus were studied. PAI was characterized by polymerase chain reaction (PCR). The presence of tdhA and tdhS genes, was determined by Southern blot. Results: Fifty three environmental strains (80%) lacked a full PAI when compared with clinical strains. In environmental strains, Southern blot and sequence analysis showed that a genetic region of 80 kilobase pairs including genes from VPA1310 to VPA1396 was missing. Conclusions: These results highlight the genetic dynamism of Vibrio parahaemolyticus
Pathogenicity island region of clinical and environmental strains of Vibrio parahaemolyticus, isolated in Chile Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales