Trypanosoma cruzi and toxoplasma gondii induce a differential MicroRNA profile in human placental explants
Author
dc.contributor.author
Medina, Lisvaneth
Author
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Castillo Rivas, Christian
Author
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Liempi, Ana
Author
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Guerrero Muñoz, Jesús
Author
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Rojas Pirela, Maura
Author
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Maya Arango, Juan Diego
Author
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Prieto, Humberto
Author
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Kemmerling Weis, Ulrike
Admission date
dc.date.accessioned
2021-05-06T22:36:51Z
Available date
dc.date.available
2021-05-06T22:36:51Z
Publication date
dc.date.issued
2020
Cita de ítem
dc.identifier.citation
Frontiers in Immunology November 2020 | Volume 11 | Article 595250
es_ES
Identifier
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10.3389/fimmu.2020.595250
Identifier
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https://repositorio.uchile.cl/handle/2250/179482
Abstract
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Trypanosoma cruzi and Toxoplasma gondii are two parasites than can be transmitted from mother to child through the placenta. However, congenital transmission rates are low for T. cruzi and high for T. gondii. Infection success or failure depends on complex parasite-host interactions in which parasites can alter host gene expression by modulating non-coding RNAs such as miRNAs. As of yet, there are no reports on altered miRNA expression in placental tissue in response to either parasite. Therefore, we infected human placental explants ex vivo by cultivation with either T. cruzi or T. gondii for 2 h. We then analyzed the miRNA expression profiles of both types of infected tissue by miRNA sequencing and quantitative PCR, sequence-based miRNA target prediction, pathway functional enrichment, and upstream regulator analysis of differentially expressed genes targeted by differentially expressed miRNAs. Both parasites induced specific miRNA profiles. GO analysis revealed that the in silico predicted targets of the differentially expressed miRNAs regulated different cellular processes involved in development and immunity, and most of the identified KEGG pathways were related to chronic diseases and infection. Considering that the differentially expressed miRNAs identified here modulated crucial host cellular targets that participate in determining the success of infection, these miRNAs might explain the differing congenital transmission rates between the two parasites. Molecules of the different pathways that are regulated by miRNAs and modulated during infection, as well as the miRNAs themselves, may be potential targets for the therapeutic control of either congenital Chagas disease or toxoplasmosis.
es_ES
Patrocinador
dc.description.sponsorship
Network of the European Union, Latin America and the Caribbean Countries on Joint Innovation and Research Activities (ERANet-LAC)
ERANet17/HLH-0142
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT
1190341
1170126
3180452