Genomic analysis of the diversity, antimicrobial resistance and virulence potential of clinical campylobacter jejuni and campylobacter coli strains from Chile
Author
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Bravo, Verónica
Author
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Katz Zondek, Assaf
Author
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Porte, Lorena
Author
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Weitzel, Thomas
Author
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Varela, Carmen
Author
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González Escalona, Narjol
Author
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BlondelI, Carlos J.
Admission date
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2021-12-14T13:55:45Z
Available date
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2021-12-14T13:55:45Z
Publication date
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2021
Cita de ítem
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PLoS Negl Trop Dis 15(2): e0009207
es_ES
Identifier
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10.1371/journal.pntd.0009207
Identifier
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https://repositorio.uchile.cl/handle/2250/183186
Abstract
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Campylobacter jejuni and Campylobacter coli are the leading cause of human gastroenteritis in the industrialized world and an emerging threat in developing countries. The incidence of campylobacteriosis in South America is greatly underestimated, mostly due to the lack of adequate diagnostic methods. Accordingly, there is limited genomic and epidemiological data from this region. In the present study, we performed a genome-wide analysis of the genetic diversity, virulence, and antimicrobial resistance of the largest collection of clinical C. jejuni and C. coli strains from Chile available to date (n = 81), collected in 2017-2019 in Santiago, Chile. This culture collection accounts for more than one third of the available genome sequences from South American clinical strains. cgMLST analysis identified high genetic diversity as well as 13 novel STs and alleles in both C. jejuni and C. coli. Pangenome and virulome analyses showed a differential distribution of virulence factors, including both plasmid and chromosomally encoded T6SSs and T4SSs. Resistome analysis predicted widespread resistance to fluoroquinolones, but low rates of erythromycin resistance. This study provides valuable genomic and epidemiological data and highlights the need for further genomic epidemiology studies in Chile and other South American countries to better understand molecular epidemiology and antimicrobial resistance of this emerging intestinal pathogen.
Author summary
Campylobacter is the leading cause of bacterial gastroenteritis worldwide and an emerging and neglected pathogen in South America. In this study, we performed an in-depth analysis of the genome sequences of 69 C. jejuni and 12 C. coli clinical strains isolated from Chile, which account for over a third of the sequences from clinical strains available from South America. We identified a high genetic diversity among C. jejuni strains and the unexpected identification of clade 3 C. coli strains, which are infrequently isolated from humans in other regions of the world. Most strains harbored the virulence factors described for Campylobacter. While similar to 40% of strains harbored mutation in the gyrA gene described to confer fluoroquinolone resistance, very few strains encoded the determinants linked to macrolide resistance, currently used for the treatment of campylobacteriosis. Our study contributes to our knowledge of this important foodborne pathogen providing valuable data from South America.
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Patrocinador
dc.description.sponsorship
Howard Hughes Medical Institute (HHMI)-Gulbenkian International Research Scholar Grant 55008749
Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
CONICYT FONDECYT 1201805
REDI170269
1191074
DICYT 022001BZ
MCMi Challenge Grants program 2018-646
FDA Foods Program Intramural Funds
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Lenguage
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en
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Publisher
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Public Library Science
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Type of license
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Attribution-NonCommercial-NoDerivs 3.0 United States
Genomic analysis of the diversity, antimicrobial resistance and virulence potential of clinical campylobacter jejuni and campylobacter coli strains from Chile