Osteoconductive properties of upsidedown bilayer collagen membranes in rat calvarial defects
Author
dc.contributor.author
Feher, Balazs
Author
dc.contributor.author
Apaza Alccayhuaman, Karol Ali
Author
dc.contributor.author
Strauss Avendaño, Franz Josef
Author
dc.contributor.author
Lee, Jung-Seok
Author
dc.contributor.author
Tangl, Stefa
Author
dc.contributor.author
Kuchler, Ulrike
Author
dc.contributor.author
Gruber, Reinhard
Admission date
dc.date.accessioned
2022-01-10T21:33:34Z
Available date
dc.date.available
2022-01-10T21:33:34Z
Publication date
dc.date.issued
2021
Cita de ítem
dc.identifier.citation
International Journal of Implant Dentistry (2021) 7:50
es_ES
Identifier
dc.identifier.other
10.1186/s40729-021-00333-y
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/183649
Abstract
dc.description.abstract
Background: Bilayer collagen membranes are routinely used in guided bone/tissue regeneration to serve as
osteoconductive scaffolds and prevent the invasion of soft tissues. It is recommended to place the membranes
with their dense layer towards the soft tissue and their porous layer towards the bony defect area. However,
evidence supporting this recommendation is lacking. This study aimed to determine whether the alignment of
bilayer collagen membranes has an effect on bone regeneration.
Methods: In two groups of ten male Sprague-Dawley rats each, a 5-mm calvarial defect was created. Thereafter,
the defect was randomly covered with a bilayer, resorbable, pure type I and III collagen membrane placed either
regularly or upside-down (i.e., dense layer towards bone defect). After 4 weeks of healing, micro-computed
tomography (μCT), histology, and histomorphometry of the inner cylindrical region of interest (4.5 mm in diameter)
were performed to assess new bone formation and the consolidation of the collagen membrane in the defect area.
Results: Quantitative μCT showed similar bone volume (median 8.0 mm3, interquartile range 7.0–10.0 vs. 6.2 mm3,
4.3–9.4, p = 0.06) and trabecular thickness (0.21 mm, 0.19–0.23 vs. 0.18 mm, 0.17–0.20, p = 0.03) between upsidedown
and regular placement, both leading to an almost complete bony coverage. Histomorphometry showed
comparable new bone areas between the upside-down and regularly placed membranes, 3.9 mm2 (2.7–5.4) vs. 3.8
mm2 (2.2–4.0, p = 0.31), respectively. Both treatment groups revealed the same regeneration patterns and spatial
distribution of bone with and without collagen fibers, as well as residual collagen fibers.
Conclusions: Our data support the osteoconductive properties of collagen membranes and suggest that bone
regeneration is facilitated regardless of membrane layer alignment.
es_ES
Patrocinador
dc.description.sponsorship
Center for Biomedical Research
es_ES
Lenguage
dc.language.iso
en
es_ES
Publisher
dc.publisher
Springer Japan KK
es_ES
Type of license
dc.rights
Attribution-NonCommercial-NoDerivs 3.0 United States